FAT10在4-硝基喹啉-1-氧化物诱导舌癌模型中的表达特点

Study on the variation of FAT10 expression during the course of SD rats tongue carcinoma induced by 4-NQO

目的:建立与人舌黏膜鳞癌相近的大鼠舌癌模型,并研究其发生发展过程中双泛素FAT10的表达变化。方法:实验组SD雄性大鼠使用含有40μg/m L的4-硝基喹啉-1-氧化物(4-NQO)饮水喂养,分别在8、16、24周时取大鼠舌体中病灶部位组织,行组织学观察;对照组大鼠正常饮用水喂养24周。使用免疫组织化学法检测FAT10、p53在舌黏膜不同程度病变组织中的表达。结果:大鼠舌黏膜经过4-NQO不同时间的刺激,可发展成不同程度的上皮异常增生以及鳞状细胞癌。从正常黏膜到不同程度的不典型增生以及最后高分化的鳞癌中,FAT10的表达强度呈逐渐增高趋势;p53在正常黏膜为阳性表达,在不典型增生中表达升高,而在癌组织中表达降低。结论:在舌癌的发生、发展过程中,FAT10可能发挥了癌基因的作用。

Objective： To establish a more realistic animal model for oral carcinogenesis and study the expression changes of double ubiquitin FAT10 in the process of development in this model. Methods：The experimental group SD rats were fed with 40 μg/mL concentration of 4-nitroquinoline1-oxide （4-NQO） dissolved in drinking water. The lesion tissue of rat tongue was collected in 8, 16 and 24 weeks respectively, and detected by histology. The rats in control group were fed with normal drinking water for 24 weeks. Immunohistochemistry was used to detect the expression of FAT10 and p53 in different tissues of the tongue mucosa. Results：Being stimulated with 4-NQO at different time, the tongue mucosa of rats developed dysplasia of varying degrees and squamous cell carcinoma. The expression of FAT10 increased gradually from normal mucosa to atypical hyperplasia and squamous cell carcinoma. The espression of p53 was positive in normal mucosa, and increased in atypical hyperplasia, but was decreased in cancer tissues. Conclusions：FAT10 might be an oncogene in the development of tongue cancer.