通心络对2型糖尿病肾病大鼠纤溶调节因子PAI-1表达的影响

Effects of Tongxinluo on PAI-1 level in rats with type 2 diabetic nephropathy

目的观察通心络对2型糖尿病肾病大鼠血流变、纤溶调节因子表达的影响。方法采用高脂饲料联合小剂量链脲佐菌素建立2型DN大鼠模型,随机分为模型组和通心络组各10只,设立10只正常SD大鼠为空白组;予通心络干预16周后,大鼠腹主动脉取血,测定空腹血糖(fasting blood glucose,FBG)、血清尿素氮(urea nitrogen,BUN)、血清肌酐(serum creatinine,Scr)、全血黏度(高切、低切)、血浆黏度、血小板聚集率、血浆纤维蛋白原、组织型纤溶酶原激活剂(tissue plasminogenemia activator,t PA)及纤溶酶原激活剂抑制剂-1(plasminogen activator inhibitor-1,PAI-1)。结果与空白组比较,模型组大鼠FBG、BUN、Scr均明显升高(P<0.05);全血黏度(高切、低切)、血浆黏度、血小板聚集率、血浆纤维蛋白原及PAI-1明显升高,t-PA明显降低。与模型组比较,通心络组BUN、Scr均明显降低(P<0.05),高、低切变率下的全血黏度、血浆黏度、血浆纤维蛋白原含量、血小板聚集率及PAI-1水平明显降低(P<0.05),但对FBG、t PA水平没有明显的影响。结论通心络可保护2型糖尿病肾病大鼠肾功能、减轻肾脏病理损伤;抗凝、降纤改善肾脏局部微循环灌注可能是其延缓DN进展的作用机制。

Objective To explore effects of Tongxinluo on hemorheology and PAI level in rats with type 2 diabetic nephropathy. Methods The rat model of type 2 diabetes mellitus was induced by injection of low-dose of streptozocin after having high-fat diet. Diabetic rats were randomly divided the model group （n= 10） arid the Tongxinluo group（ n = 10）. Normal SD rats （n = 10） were used as the control group. After intervention with Tongxinluo for 8 weeks, fasting blood glucose （FBG）, serum creatinine （Scr）, serum urea nitrogen （BUN）, tissue plasminogen activator （t-PA）, plasminogen activator inhibitor type-1 （PAI-1） and hemorheoiogy indexes were detected. Results Compared with control group, FBG, SCr, BUN, hemorheology indexes in the model group were significantly increased while the PAI-1 was decreased （P〈 0.05 ）. Compared with model group, BUN and Scr were significantly decreased （ P 〈 0. 05 ） ,hemorheology indexes and PAI-1 levels were significantly decreased （ P〈0. 05）, but the FBG and tPA level had no obvious influence. Conclusion TongXinLuo can protect renal function and alleviate renal pathological damage in type 2 diabetic nephropathy rats. Anticoagulation, defibringen and improving local microcirculation perfusion may be the mechanisms of delaying DN progression.