子宫内膜癌细胞表达纤维蛋白原的检测

Detection of expression of fibrinogen in endometrial carcinoma cells

目的探讨子宫内膜癌细胞是否表达纤维蛋白原。方法纳入于医院接受子宫切除手术的16例子宫内膜癌患者(内膜癌组),同期4例良性子宫肿瘤切除的病例(对照组),采用Western blot和q RT-PCR实验,分别检测各子宫内膜组织标本中纤维蛋白原蛋白和三链(a、b、g)m RNA表达水平。结果 Western blot检测结果显示,81.25%(13/16)的内膜癌组标本存在纤维蛋白原蛋白表达,而对照组未观察到其表达;q RT-PCR检测结果显示,内膜癌组有7例标本(43.8%)存在至少一个纤维蛋白原链m RNA的表达,而对照组均未观察到其表达。表达纤维蛋白原m RNA的全部7例标本在Western blot检测中均呈较高水平表达。结论绝大多数子宫内膜癌细胞具备表达纤维蛋白原的能力,是子宫内膜癌高纤维蛋白原血症的重要来源之一。

Objective To explore whether endometrial carcinoma cells express fibrinogen or not. Methods A total of 16 patients with endometrial carcinoma to receive hysterectomy in our hospital（ ＂endometrial carcinoma group＂） and another four patients to receive Benign hysterectomy in the same period（ ＂the control group＂） were selected. Western Blot and q RT-PCR experiments were respectively conducted to detect the expression levels of the fibrinogen and triple-stranded（a, b and g） m RNA in specimens of endometrium. Results The results of Western Blot detection showed that 81.25%（13/16） of endometrial carcinoma specimens had the expression of fibrinogen, while the expression thereof was not observed in the control group; the results of q RT-PCR detection showed that seven specimens（43.8%） in the endometrial carcinoma group had the expression of at least one fibrinogen chain m RNA,but such expression was not observed in the control group. All of the seven specimens with the expression of fibrinogen m RNA were at high expression levels in Western blot detection. Conclusion Most endometrial carcinoma cells are capable of expressing fibrinogen, which is an important source of endometrial carcinoma hyperfibrinogenemia.