腹腔灌注化疗联合深部热疗对卵巢癌合并腹水患者腹水中恶性分子表达的影响

Influence of intraperitoneal perfusion chemotherapy combined with deep hyperthermia on the malignant molecule expression in ascites of patients with ovarian cancer complicated with ascites

目的:探讨腹腔灌注化疗联合深部热疗对卵巢癌合并腹水患者腹水中恶性分子表达的影响。方法:2015年3月~2017年1月间在本院就诊的80例卵巢癌合并腹水患者,回顾治疗方案并分为对照组43例、研究组37例。对照组接受腹腔灌注化疗,研究组接受腹腔灌注化疗联合深部热疗。对比治疗前后两组患者腹水中增殖、侵袭、自噬等恶性分子表达量的差异。结果:治疗前,两组腹水中增殖、侵袭、自噬等恶性分子表达量的差异无统计学意义。治疗后,研究组腹水中增殖基因TCEAL7 mRNA的表达量高于对照组,Clusterin、HOTAIR、ROCK、TNFAIP8mRNA的表达量低于对照组;腹水中侵袭基因DUSP10mRNA的表达量高于对照组,MTA1、Nek2、Stathmin、IFITM1mRNA的表达量低于对照组;腹水中自噬基因LC3-Ⅱ、Beclin1、PTEN mRNA的表达量高于对照组。结论:卵巢癌合并腹水患者接受腹腔灌注化疗联合深部热疗,可有效均衡腹水中增殖、侵袭、自噬基因的表达,最终降低肿瘤恶性程度。

Objective:To explore the influence of intraperitoneal perfusion chemotherapy combined with deep hyperthermia on the malignant molecule expression in ascites of patients with ovarian cancer complicated by ascites.Methods:80 patients with ovarian cancer complicated by ascites who were treated in this hospital between March 2015 and January 2017 were retrospectively analyzed and divided into the control group(n=43)and the study group(n=37).Control group received intraperitoneal perfusion chemotherapy and study group underwent intraperitoneal perfusion chemotherapy combined with deep hyperthermia.The differences in the expression of proliferation,invasion,autophagy and other malignant molecules in ascites were compared between the two groups before and after treatment.Results:Before treatment,the differences in the expression of proliferation,invasion,autophagy and other malignant molecules in ascites were not statistically significant between the two groups.After treatment,proliferation gene TCEAL7 mRNA expression in ascites of study group was higher than that of control group whereas Clusterin,HOTAIR,ROCK and TNFAIP8 mRNA expression were lower than those of control group;invasion gene DUSP10 mRNA expression in ascites was higher than that of control group whereas MTA1,Nek2,Stathmin and IFITM1 mRNA expression were lower than those of control group;autophagy genes LC3-Ⅱ,Beclin1 and PTEN mRNA expression in ascites were higher than those of control group.Conclusion:Intraperitoneal perfusion chemotherapy combined with deep hyperthermia can effectively balance the expression of proliferation,invasion and autophagy genes in ascites,and ultimately reduce the malignancy of the tumor in patients with ovarian cancer complicated by ascites.