血清GP73、SOD、GPC3含量与肝癌病灶内细胞增殖、血管新生的相关性研究

Correlation of serum GP73,SOD and GPC3 contents with cell proliferation and angiogenesis in liver cancer lesion

目的:研究血清GP73、SOD、GPC3含量与肝癌病灶内细胞增殖、血管新生的相关性。方法:选择2014年6月~2017年2月期间在江汉油田总医院诊断为原发性肝癌的患者作为肝癌组,同期在江汉油田总医院体检的健康者作为对照组,采集两组受试者的血清并测定GP73、SOD、GPC3的含量;采集肝癌组患者的肝癌病灶和癌旁病灶,测定细胞增殖分子及血管新生分子的表达量。结果:肝癌组患者血清中GP73、GPC3的含量显著高于对照组,SOD的含量显著低于对照组;肝癌组患者肝癌病灶内DNMT3B、STC2、SIRT6、LETM1、EphB4、SULT2B1、HIF-1α、VEGF、Ang-2、HGF、TGF-β1的蛋白表达量显著高于癌旁病灶;肝癌组患者肝癌病灶内DNMT3B、STC2、SIRT6、LETM1、EphB4、SULT2B1、HIF-1α、VEGF、Ang-2、HGF、TGF-β1的蛋白表达量与血清中GP73、GPC3的含量呈正相关,与血清中SOD的含量呈负相关。结论:肝癌患者血清中GP73、SOD、GPC3含的变化与肝癌病灶内细胞增殖、血管新生密切相关。

Objective:To study the correlation of serum GP73,SOD and GPC3 contents with cell proliferation and angiogenesis in liver cancer lesion.Methods:Patients who were diagnosed with primary liver cancer in Jianghan Oilfield General Hospital between June 2014 and February 2017 were selected as liver cancer group,and healthy subjects who received physical examination in Jianghan Oilfield General Hospital during the same period were selected as control group.Serum was collected from two groups of subjects to determine the contents of GP73,SOD and GPC3;liver cancer lesion and adjacent lesion were collected from liver cancer group to determine the expression of cell proliferation molecules and angiogenesis molecules.Results:Serum GP73 and GPC3 levels of liver cancer group were obviously higher than those of control group while SOD content was obviously lower than that of control group;DNMT3 B,STC2,SIRT6,LETM1,EphB4,SULT2 B1,HIF-1α,VEGF,Ang-2,HGF and TGF-β1 protein expression levels in liver cancer lesion of liver cancer group were significantly higher than those in adjacent lesion;DNMT3 B,STC2,SIRT6,LETM1,EphB4,SULT2 B1,HIF-1α,VEGF,Ang-2,HGF and TGF-β1 protein expression levels in liver cancer lesion of liver cancer group were positively correlated with serum GP73 and GPC3 levels,and negatively correlated with serum SOD level.Conclusion:The changes of GP73,SOD and GPC3 levels in the serum of patients with liver cancer are closely related to the cell proliferation and angiogenesis in liver cancer lesion.