木犀草素抑制结肠癌HT-29细胞增殖、迁移及上皮间质转化的实验研究

Luteolin inhibits the colon cancer HT-29 cell proliferation,migration and epithelial-mesenchymal transition:an experimental study

目的:研究木犀草素对结肠癌HT-29细胞增殖、迁移及上皮间质转化的调节作用。方法:培养结肠癌HT-29细胞并随机分为两组,对照组用不含药物的无血清培养基处理,LUT组用含有木犀草素的无血清培养基处理。处理24h后,收集细胞、抽提RNA后采用荧光定量PCR法测定增殖基因、迁移基因、上皮间质转化基因的mRNA表达量。结果:木犀草素处理24h后,LUT组细胞中Lrig1、TSPYL5、Bim、SOX15、DLC1的mRNA表达量显著高于对照组,RPS15a、Bad、TRPV5、TRPV6、PLD2、IBP、SphK1、FAK、Vimentin、N-cadherin的mRNA表达量显著低于对照组。结论:木犀草素对结肠癌HT-29细胞增殖、迁移及上皮间质转化具有抑制作用。

Objective:To study the regulating effect of luteolin on colon cancer HT-29 cell proliferation,migration and epithelial-mesenchymal transition.Methods:Colon cancer HT-29 cells were cultured and randomly divided into two groups,control group were treated with serum-free medium without drugs and LUT group were treated with serum-free medium containing luteolin.After 24 hours of treatment,cells were collected to extract RNA,and then fluorescent quantitative PCR method was used to determine the mRNA expression of proliferation genes,migration genes and epithelial-mesenchymal transition genes.Results:After 24 hours of luteolin treatment,Lrig1,TSPYL5,Bim,SOX15 and DLC1 mRNA expression in LUT group were significantly higher than those in control group while RPS15 a,Bad,TRPV5,TRPV6,PLD2,IBP,SphK1,FAK,Vimentin and N-cadherin mRNA expression were significantly lower than those in control group.Conclusion:Luteolin has inhibiting effect on colon cancer HT-29 cell proliferation,migration and epithelial-mesenchymal transition.