增强CT定量参数与胰腺癌病灶内增殖、侵袭基因表达量的相关性

The correlation of contrast-enhanced CT quantitative parameters with proliferation and invasion gene expression in pancreatic cancer

目的:探讨增强CT定量参数与胰腺癌病灶内增殖、侵袭基因表达量的相关性。方法:选取2015年5月~2017年5月间收治的胰腺癌患者100例作为胰腺癌组,同期在本院接受手术治疗的坏死性胰腺炎患者48例作为胰腺炎组。对比两组胰腺增强CT值差异,病灶组织增殖相关基因、侵袭相关基因表达的差异,采用Pearson检验评估胰腺癌组织CT值与增殖侵袭基因表达量的相关关系。结果:胰腺癌组织增强CT值低于胰腺炎组;胰腺癌组病灶组织中增殖相关基因Bmi-1、Ezrin、FOXC1 mRNA的表达量高于胰腺炎组,EphB2、RGC-32mRNA的表达量低于胰腺炎组;胰腺癌组病灶组织中侵袭相关基因FHL1、JDP2、Let-7f、TFPI-2mRNA的表达量低于胰腺炎组,Twist、HOXB7 mRNA的表达量高于胰腺炎组。Pearson检验提示胰腺癌增强CT值与病灶组织增殖、侵袭基因表达量直接相关。结论:胰腺癌组织增强CT值较正常胰腺组织低,且具体CT值与肿瘤增殖、侵袭相关基因表达量直接相关,可作为早期诊断疾病、判断病情严重程度的简便、可靠手段。

Objective:To discuss the correlation of contrast-enhanced CT quantitative parameters with proliferation and invasion gene expression in pancreatic cancer.Methods:100 patients with pancreatic cancer who were treated in the hospital between May 2015 and May 2017 were selected as pancreatic cancer group,and 48 patients with necrotic pancreatitis who received surgical treatment in the hospital during the same period were selected as pancreatitis group.Differences in pancreatic contrastenhanced CT value as well as the expression of proliferation-related genes and invasion-related genes in lesion tissue were compared between the two groups,and Pearson test was used to evaluate the correlation of pancreatic cancer tissue CT value with proliferation and invasion gene expression.Results:Contrast-enhanced CT value of pancreatic cancer tissue was lower than that of pancreatitis tissue;proliferation-related genes Bmi-1,Ezrin and FOXC1 mRNA expression in lesion tissue of pancreatic cancer group were higher than those of pancreatitis group while EphB2 and RGC-32 mRNA expression were lower than those of pancreatitis group;invasion-related genes FHL1,JDP2,Let-7 fand TFPI-2 mRNA expression in lesion tissue of pancreatic cancer group were lower than those of pancreatitis group while Twist and HOXB7 mRNA expression were higher than those of pancreatitis group.Pearson test showed that the contrast-enhanced CT value of pancreatic cancer was directly correlated with the proliferation and invasion gene expression in lesion tissue.Conclusion:Contrast-enhanced CT value of pancreatic cancer tissue was lower than that of normal pancreas tissue,and the specific CT value was directly correlated with the tumor proliferation and invasion-related gene expression,and can be used as a simple and reliable method for early diagnosis of disease and judgement of illness severity.