住院患者鼻咽灌洗液标本中呼吸道感染病毒检测及耐药性分析

Analysis of the pathogen of respiratory tract infection and drug resistance in nasopharyngeal lavage fluid samples in hospitalized patients

目的对住院患者鼻咽灌洗液标本中的呼吸道感染病毒进行检测及耐药性分析。方法选取2016年该院住院患者3 091例,其中呼吸道感染患者556例,采集受试对象鼻咽灌洗液进行研究。采用免疫荧光法检测呼吸道病毒抗原谱,并进行病毒耐药性分析。采用SPSS17.0统计软件包完成数据处理。结果呼吸道感染病毒检出阳性率为17.99%,其中11月最高,阳性率为35.63%,与其他月比较差异有统计学意义(P<0.05);阳性率超过全年平均水平的高发月为10―12月。病毒分布特征总体以呼吸道合胞病毒阳性率最高,进一步对该院呼吸道感染阳性率超过全年平均水平的高发月(10―12月)进行深入分析,结果提示,此阶段18岁以下患者病毒检出阳性率为32.61%,18~60岁者为30.71%,60岁以上者为28.62%,3个年龄段阳性率及男、女性之间的差异均无统计学意义(P>0.05)。58株A3型流感病毒中检出43株对金刚烷胺耐药,总耐药率为74.14%。结论病毒分布特征总体以呼吸道合胞病毒阳性率最高,且在儿童患者人群中占比最高。

Objective To analyze the pathogens of respiratory tract infection in nasopharyngeal lavage fluid samples of inpatients and drug resistance.Methods In this study,3 091 hospitalized patients were enrolled in this study.556 cases of positive respiratory tract infection were detected and the samples of nasopharyngeal lavage fluid were collected.Immunofluorescence method was used to detect the respiratory virus antigen spectrum and the drug resistance was analyzed.SPSS17.0 statistical software package was used to complete the data processing.Results The positive rate of respiratory infection was 17.99% in hospitalized patients in 2016,with the highest in November(35.63%),and the difference was statistically significant(P<0.05).The positive rates in October,November and December were higher than the average level.As for the distribution of the virus in general,respiratory syncytial virus had the highest positive rate.Further anaylsis was conducted on the positive rate of respiratory tract infection in our hospital in October,November and December in which the positive rate was higher than the annual average level,and the results suggest that the positive rate for virus detection of patients under 18 years old in this stage was 32.61%,30.71%for the patients at the age of18-60 and 28.62%for the patients above 60 years old.There were no statistically significant differences in three groups and between the two sexes.In this study,43 strains of amantadine resistance were detected in 58 strains of A3 influenza virus,and the total resistance rate was 74.14%.Conclusion The positive rate of respiratory tract infection in hospitalized patients was 17.99%,the highest month was November,and the high month was 10-12 months.As for the distribution of the virus in general,the positive rate of respiratory syncytial virus is the highest and it occupies the highest position in the population of children.