雷公藤氯内酯醇对血管性痴呆模型大鼠海马炎症因子及β淀粉样蛋白表达的影响

Effects of tripchlorolide on the expression of inflammatory factors and β-amyloid protein in the hippocampus in vascular dementia rats

目的观察雷公藤氯内酯醇对血管性痴呆(VD)模型大鼠海马区核因子-κB(NF-κB)/环氧化酶-2(COX-2)炎性通路及β样淀粉样蛋白(Aβ)的影响。方法按照体重将大鼠随机分为3组:假手术组、模型组、实验组,每组10只。实验组和模型组大鼠以永久性双侧颈总动脉结扎法(2-VO)建立大鼠血管性痴呆模型。实验组每日腹膜腔内注射含雷公藤氯内酯醇(1μg·kg^(-1)·d^(-1))的0.1%二甲基亚砜(DMSO),模型组和假手术组每日腹膜腔内注射0.1%DMSO,连续4周。以免疫印迹法测定Aβ、NF-κB p65及COX-2蛋白水平。结果海马区Aβ蛋白条带灰度值:假手术组、模型组与实验组分别为0.17±0.05,1.46±0.06,0.57±0.04。海马区NF-κB p65蛋白条带灰度值:假手术组、模型组与实验组分别为0.12±0.03,0.86±0.04,0.37±0.04。海马区COX-2蛋白条带灰度值:假手术组、模型组与实验组分别为0.23±0.04,1.26±0.05,0.73±0.04。与假手术组比较,模型组的上述3种指标表达均增高,差异均有统计学意义(均P<0.001);与模型组比较,实验组的上述3种指标表达降低,差异有均统计学意义(均P<0.001)。结论雷公藤氯内酯醇可能通过发挥抗炎作用,从而减少血管性痴呆模型大鼠海马Aβ沉积。

Objective To investigate the effects of tripchlorolide on the accumulation of nuclear factor - κb （ NF - κB ）, cyclooxygenase - 2 （ COX - 2）, β - amyloid protein （ All ） in the hippocampus in vascular dementia （VD） rats. Methods Male Sprague - Dawley （SD） rats were randomly assigned into three groups （ 10 rats per group） ： sham - operated group, model group and experimental group. The bilateralcornmoil carotid arteries ligation （2 -VO ） models were created in model and experimental rats. Tripchlorolide were dissolved in sterile 1% dimathyl sulfoxide （ DMSO ） and were administered （ 1 μg · kg- 1 d-1 ） by intraperitoneal injection daily for 4 weeks in experimental group. In sham - operated and model group rats were given DMSO in the same volume. The expression of Aβ, NF- κB, COX- 2 in the brain were detected by Western blot. Results Aβ protein bands of gray value in rat brain campus in sham - operated group, model group and experimental group were 0.17 ±0.05,1.46 ±0.06,0.57 ±0.04. NF- κB p65 protein bands of gray value in rat brain campus in sham -operated group, modelgroup and experimental group were 0. 12 ±0.03,0. 86 ±0.04,0.37 ±0.04. COX -2 protein bands of gray value in rat brain campus in sham - operated group, model group and experimental group were 0. 23 ± 0. 04, 1.26 ± 0.05, 0. 73 ±0.04. Compared with sham- operated group,the three indexes in model group were increased significantly（ all P 〈 0.001 ）. Compared with model group, the three indexes in experimental group were decreased significantly （all P 〈0. 001 ）. Conclusion Tripchlorolide exhibits anti - inflammatory and attenuates brain Aβ3 deposition in 2 - VO rats.