贵州糯质小麦的分子标记辅助育种研究

Application of Molecular-assisted Seletion of Wx Gene for Identification of Guizhou Waxy Wheat

糯性小麦具有优异的酿酒特性。目前贵州省内缺乏具有推广价值的糯性小麦,利用分子标记辅助育种,可有效提高选育适合贵州生态气候的糯性小麦新材料的效率。本研究利用与小麦糯质基因(Wx-A1a,Wx-B1a和Wx-D1a)连锁的特异分子标记,对俞糯麦×0117的F2代群体进行Wx基因位点扫描鉴定。结果发现:在显著水平p>0.05时,3个糯质基因位点在F2代中的分离比例符合预期比例。在56个F2代分离群体中,3个基因位点都存在的材料有4株(7.14%);单缺失Wx-A1a或Wx-B1a或Wx-D1a基因位点的材料编号分别为5(8.93%)、8(14.29%)和19(33.93%);Wx-A1a和Wx-B1a基因位点同时缺失的材料为5株(8.93%);Wx-A1a和Wx-D1a基因位点同时缺失材料为4株(7.14%);Wx-B1a和Wx-D1a基因位点同时缺失材料为11株(19.64%);3个基因位点都缺失的全糯质单株未发现。

Waxy wheat has excellent characteristics in brewing, but greatly 1acked applications in agri- cultural industry.It is great significance that breeding new varieties （lines） with good comprehensive traits to suit Guizhou ecological climate. In this study,Wx gene locus was used to identify F2 popu1a- tions of Yuyou wheatX 0117 using the specific molecu1ar markers linked to wheat waxy genes （Wx- A1a, Wx-B1a and Wx-D1a）.The results showed that the ratio of the three waxy genes in the F2 gen- eration was in accordance with the expected ratio at the significant level of p〉0.05.4 p1ants have all of the three Wx genes,the number of p1ants 1acked of Wx-A1a ,Wx-B1a or Wx-D1a were 5,8 and 19 re- spectively;5 p1ants 1acked both Wx-A1a and Wx-B1a;4 p1ants 1acked both Wx-A1a and Wx-D1a ;11 p1ants 1acked both Wx-B1a and Wx-D1a ;No p1ants 1acked these three Wx genes.