诺氟沙星在纳米银线基底上的SERS光谱检测

The SERS Spectra Detection of Norfloxacin on Silver Nanowires Substrate

本文以硝酸银为银源,通过多元醇法制备纳米银线,利用扫描电子显微镜、紫外可见分光光度计对纳米银线进行表征,成功制备出直径、长度均一的纳米银线。使用激发波长为785nm的手持式拉曼光谱仪对诺氟沙星溶液进行检测。结果表明该基底具有很好的SERS效果,增强因子达到1.44×107。检测10-3~10-8 mol/L浓度的诺氟沙星溶液时,以浓度和特征峰强度作直线拟合,发现具有较好的线性相关,相关系数R2为0.94101;为进一步考察纳米银线在玻璃片上的分散性,在诺氟沙星(10-3 mol/L)样品的基底上随机取6个点,采集光谱信号。最后,重复检测6个诺氟沙星溶液(10-5 mol/L)样品的拉曼光谱,检验光谱的重现性。实验结果表明,纳米银线在玻璃片上分散性较好,在检测诺氟沙星时体现了较好的重现性。

In this paper, silver nanowires（AgNWs） were synthesized by a polyol process using AgNO3 as silver source,characterized by scanning electron microscope and UV-Vis Spectro- photometer. It shows that the diameter and length of the AgNWs are uniform. The Raman signal were obtained using a handheld Raman spectrometer equipped with a 785nm incident laser. The results show that the base has a good SERS effect, and the enhancement factor is achieved 1.44 × 107 , When the concentration of 10-3- 10 8 mol/L norfloxacin solution was measured, the concentration and intensity of the characteristic peaks were linearly fitted and found to have a good linear correlation. The correlation coefficient R2 was 0. 941 01. In order to detect the dispersion of AgNWs on glass,six points were randomly picked on the substrate to obtain the Raman signal of norfloxacin solution（10.3mol/L）. To test the reproducibility of the spectrum, six Raman spectra of norfloxacin solution（10-Stool/L） samples were detected. The experimental results show that the AgNWs have the better dispersion on the glass and a good spectrum reproducibility in testing norfloxacin.