紫外分光光度法在卡马西平中毒诊断中的临床意义

Clinical significance of ultraviolet spectrophotometry in the diagnosis of carbamazepine poisoning

【目的】探讨建立血清中卡马西平定性、定量的紫外分光光度法测定,为临床卡马西平药物中毒提供诊疗依据。【方法】取0.5 ml血清碱化后加乙酸乙酯5.0 ml充分震荡萃取,静置5 min,以4 000 r/min高速离心,取乙酸乙酯层置于尖底玻璃试管中氮气吹干,加200μl无水乙醇溶液,取50μl于微量比色池中紫外扫描。【结果】血清中卡马西平最大吸收峰285.5 nm,浓度在3.0~30.0μg/ml内呈线性,回归方程Y=0.0884X+0.0566,相关系数r=0.9990。最低检出浓度:1.0μg/ml,方法回收率为87.8%~102.6%;相对标准差(RSD%)为:3.8%~4.7%。日内、日间相对标准差(RSD%)分别为2.67%~4.85%和2.77%~4.80%。【结论】紫外分光光度法操作简便、分析快速,结果准确,为临床诊断卡马西平中毒提供了一个简便准确的检测方法。

[Objective] To explore the qualitative and quantitative methods for carbamazepine determination in serum by ultraviolet spectrophotometry, and provide reliable evidence for clinical diagnosis of carbamazepine poisoning. [ Methods ] The 0.5 ml serum was alkalized, mixed with 5.0 ml ethyl acetate for full shock extraction. After 5 min, the mixture was centrifuged at 4 000 r/min. The ethyl acetate layer was collected and blow-dried with nitrogen in the tube with sharp bottom. Finally, 200 μl 100% ethanol was used to dissolve the pellet and 50 μl of the solution was loaded in the microcuvette for ultraviolet scanning. [ Results ] The maximum absorption peak of carbamazepine in the serum was 285.5 nm. A linear relationship was found when the concentration of carbamazepine in the serum ranged from 3.0 μg/ml to 30.0 pg/ml. The regression equation was as follows： Y=0.0884x ＋0.0566 （t=0.9990）. The minimum detectable concentration was 1.0 μg/ml and the recovery rate ranged from 87.8% to 102.6%. Besides, the relative standard deviation （RSD %） ranged from 3.8% to 4.7% while the intraday RSD ranged from 2.67% to 4.85% and the interday RSD was from 2.77% to 4.80%. [ Conclusion ] Ultraviolet spectrophotometry is easy for operation, efficient for analysis and accurate for results. Therefore, it provides a simple and accurate way for the clinical diagnose of carbamazepine poisoning.