海洋厌氧氨氧化菌处理含海水污水的基质抑制及其动力学特性

Substrate Inhibition and Kinetic Characteristics of Marine Anaerobic Ammonium Oxidizing Bacteria Treating Saline Wastewater

采用ASBR反应器通过改变单一基质浓度分别研究了NH_4^+-N和NO_2^--N对海洋厌氧氨氧化菌脱氮效能的影响及其动力学特性.结果表明,保持进水NO_2^--N为105.6 mg·L^(-1),当进水NH_4^+-N浓度提高至1 200 mg·L^(-1)时,海洋厌氧氨氧化反应器仍保持较好的脱氮能力,未受到明显的抑制作用,NO_2^--N的去除率稳定在80.70%左右;当进水NO_2^--N浓度提高至265.6mg·L^(-1)时,反应器开始受到明显的抑制作用,NH_4^+-N的去除率下降至63.01%左右,随着进水NO_2^--N浓度继续提高至305.6mg·L^(-1)时,NH_4^+-N的去除率进一步下降至43.93%左右.利用Haldane模型和Aiba模型拟合NH_4^+-N和NO_2^--N抑制作用的动力学特性,得到了NRRmax、KS、Ki这3个动力学参数及出水基质浓度与总氮容积负荷(TNRR)之间的关系,根据进一步分析可知,Haldane模型更适合描述NH_4^+-N抑制作用下的动力学特性,Aiba模型更适合描述NO_2^--N抑制作用下的动力学特性,并得到NH_4^+-N和NO_2^--N的出水抑制浓度分别为3 893.625 mg·L^(-1)和287.208 mg·L^(-1),为海洋厌氧氨氧化菌处理含海水污水提供了理论依据.

An anaerobic sequencing batch reactor （ASBR） was used to study substrate inhibition and kinetic characteristics of marine anaerobic ammonium oxidizing bacteria （MAAOB） treating saline wastewater. The results indicated that when ammonia increased to 1200 mg·L - 1 , the MAAOB still maintained good nitrogen removal capability, though there was a slight inhibitory effect. At the same time, nitrite nitrogen removal efficiency was stable at about 80. 70% . When nitrite increased to 265. 6 mg·L - 1 , the MAAOB were inhibited obviously, and ammonia nitrogen removal efficiency decreased to about 63. 01% . When influent nitrite concentration increased to 305. 6 mg·L - 1 , the removal rate of ammonia nitrogen further decreased to 43. 93% . The kinetic characteristics resulting from inhibition of the MAAOB were simulated by the Haldane model and Aiba model. Three parameters, TNRRmax , KS , and Ki , and the relationship between effluent substrate concentration and total nitrogen loading （TNRR） were evaluated. Based on further analysis, the Haldane model was more suitable for describing dynamic characteristics resulting from NH ＋4 -N inhibition, while the Aiba model was more suitable for describing the dynamic characteristics resulting from NO -2 -N inhibition. The predicted effluent inhibitory concentrations of NH ＋4 -N and NO -2 -N were 3893. 625 mg·L - 1 and 287. 208 mg·L - 1 , respectively. The results could provide a theoretical basis for saline wastewater treatment by MAAOB.