接种单一/混合污泥对厌氧氨氧化反应器快速启动的影响

Effect of Seeding Single/Mixed Sludge on Rapid Start-up of an ANAMMOX Reactor

在两组SBR反应器R0、R1中分别接种单一类型反硝化颗粒污泥和反硝化颗粒污泥与好氧硝化污泥的混合污泥(体积比为2∶1)来启动厌氧氨氧化,旨在探求不同接种污泥对厌氧氨氧化反应器快速启动的影响.结果表明,R0用时64 d成功启动厌氧氨氧化,总氮去除负荷为0.26 kg·(m^3·d)^(-1),R1用时47 d,总氮去除负荷为0.30 kg·(m^3·d)^(-1),比R0缩短了17 d;在富集培养阶段,R1中红色污泥大量出现,系统厌氧氨氧化特征比R0更加明显;反应器启动成功后,R0的化学计量比为1.20和0.34,R1的化学计量比为1.26和0.21,比R0更接近理论值1.32和0.26,R0中污泥的MLSS和MLVSS分别恢复到初始种泥的51%(4.2 g·L^(-1))和38%(2.3 g·L^(-1)),R1中污泥的MLSS和MLVSS分别恢复到初始种泥的54%(4.4 g·L^(-1))和42%(2.6 g·L^(-1)),高于R0,可以推测,R1驯化过程中厌氧氨氧化菌(AnAOB)增殖速率比R0更快.采用混合污泥作为接种污泥能够加速厌氧氨氧化的启动进程,且启动成功之后系统的脱氮性能更加稳定.

The experiment explored the effect of different seeding sludge on the rapid start-up of an anaerobic ammonium oxidation （ANAMMOX） reactor by seeding a single type of denitrified granular sludge and a mixed sludge composed of denitrified granular sludge and aerobic nitrification sludge （the volume ratio of the mixed sludge was 2： 1） in two sequencing batch reactors （SBR）, R0 and R1, respectively. The results indicated that R0 was started up successfully on day 64 with a nitrogen removal rate （NRR） of 0. 26 kg·（m3·d） - 1 , while R1 was started up by day 47 with a NRR of 0. 30 kg·（m3·d） - 1 , which was shorter than R0 by 17 d. In the enrichment stage, reddish sludge appeared in R1, and the characteristics of anaerobic ammonium oxidation of the system were more obvious than in R0. After the reactor was started up successfully, the stoichiometric ratio of R0 was 1. 20 and 0. 34, respectively, and the stoichiometric ratio of R1 was 1. 26 and 0. 21, which was closer to the theoretical values of 1. 32 and 0. 26. The mixed liquor suspended solids （MLSS） and mixed liquor volatile suspended solids （MLVSS） of R0 were restored to 51% （4. 2 g·L - 1 ） and 38%（2. 3 g·L - 1 ）of the initial seeding sludge, respectively, while the MLSS and MLVSS of R1 were restored to 54% （4. 4 g·L - 1 ） and 42% （2. 6 g·L - 1 ）,which was higher than R0. It can be speculated that the proliferation rate of AnAOB in R1 was faster than in R0. Seeding mixed sludge can accelerate the start-up process of anaerobic ammonium oxidation with more stable N-removal performance.