摘要
对Gene Bank数据库中Clematis fusca var.coreana叶绿体全基因组进行了分析,以此为基础设计出11对叶绿体SSR引物,并进一步研究了11对叶绿体SSR引物在铁线莲属其他野生种类和栽培品种中的通用性。结果表明,C.fusca var.coreana叶绿体基因组内共有67个SSR位点,主要分布于1 088~41 445 bp和50 135~127 217 bp,以A/T单碱基重复为主;设计所得11对cpSSR引物在铁线莲野生种类和栽培品种中均表现出良好的通用性,在55个铁线莲样本中扩增等位基因数(Na)平均为10.91,有效等位基因数(Ne)平均为5.40,主等位基因频率(MAF)平均为0.31,多态信息含量(PIC)平均为0.79。另外,基于cpSSR数据,对55个样本的UPGMA聚类结果进行了初步讨论。
The whole chloroplast genome sequence of Clematis fusca var. coreana from Gene Bank database was analyzed,and 11 cpSSR primer pairs were designed based on that. In addition,the transferability of the 11 pairs of cpSSR primer in different species and cultivars of Clematis were studied. The results showed that 67 cpSSRs were distributed across the whole chloroplast genome,and most of them located in the area 1 088-41 445 bp and 50 135-127 217 bp,which generally consisted of a single base A/T. All of the 11 pairs of cpSSR primer could be successfully cross-amplified in different species and cultivars of Clematis,which indicated that the 11 primer pairs for cpSSR markers had good transferability in Clematis. The average number of alleles( Na) was 10. 91,effective number of alleles( Ne) was 5. 40,major allele frequency( MAF) was 0. 31,and polymorphism information content( PIC) was 0. 79. In addition,clustering result of UPGMA of 55 samples based on cpSSR data was discussed.
出处
《浙江农业学报》
CSCD
北大核心
2017年第12期2023-2031,共9页
Acta Agriculturae Zhejiangensis
基金
浙江省农业(林木)新品种选育重大科技专项子课题(2016C02056-13-4)
浙江农林大学学生科研训练项目(104-2013200015)
