拟穴青蟹miR-34靶基因预测及其生物信息学分析

Prediction and bioinformatic analysis of miR-34 target genes in Scylla paramamosain

MiR-34是一类在进化过程中高度保守的miRNA家族,参与机体的许多重要生命活动。本研究首先比较本实验室构建的拟穴青蟹miRNA数据库中的miR-34(spa-miR-34)和通过miRBase获得的多个物种的miR-34的序列特征,然后应用RNAhybrid和Segal Lab两种在线工具预测spa-miR-34的靶基因,用blast2go软件对这些靶基因进行功能注释和信号通路富集分析。结果表明,spa-miR-34可能的靶基因有66个,这些基因的功能主要包括氧化还原、磷酸化、钙离子跨膜运输、转录等生物学过程;信号通路主要富集于精氨酸和脯氨酸代谢、抗生素生物合成、苯丙素生物合成、糖酵解和糖异生等合成代谢通路中。spa-miR-34预测的靶基因所涉及的基因家族和通路亦包括CHH家族、ERK通路、细胞周期和UPP通路、SUMO通路。此外,spa-miR-34预测的靶基因还有蜕皮激素受体,推测spa-miR-34在生殖、蜕皮及氨基酸等物质代谢方面具有重要作用。本研究为进一步了解spa-miR-34在拟穴青蟹中的作用提供了重要参考资料。

MiR-34 is a family of microRNAs that is highly conserved in evolution and is involved in many important life activities.In this study,the sequences characteristics between spa-miR-34 of Scylla paramamosain from the database constructed by our laboratory and miR-34 of other animals from the miRBase were compared,and then the spa-miR-34 target genes were predicted by two online tools: RNAhybrid and Segal Lab.The functional annotation and signal pathway enrichment of these target genes were analyzed by using blast2 go software.The results showed that the number of the predicted pa-miR-34 target gene was 66,and the functions of these genes are mainly distributed in the biological processes of oxidation-reduction,phosphorylation,calcium ion transmembrane transport,transcription,etc.Signal pathways related to these genes are mainly concentrated in the metabolism of arginine and proline metabolism,antibiotic biosynthesis,phenylpropanoid biosynthesis,glycolysis and glycogendysis,and other synthetic metabolic pathways.The predicted gene family and pathways of the spa-miR-34 target genes also include CHH family,ERK pathway,cell cycle and UPP pathway,and SUMO pathway.In addition,ecdysteroid receptor gene was also one of the predicted target genes.It is speculated that spa-miR-34 plays an important role in the reproduction,molt and the metabolism of amino acids and other substances.This study provides an important reference for further understand the role of spa-miR-34 in S.paramamosain.