虫草素对非小细胞肺癌细胞株H358增殖行为及相关基因表达的影响

Effect of cordycepin on non-small cell lung cancer cell line H358 proliferation and related gene expression

目的:研究虫草素对非小细胞肺癌细胞株H358增殖行为及相关基因表达的影响。方法:选择C57BL/6小鼠作为实验动物,培养非小细胞肺癌细胞株H358后建立移植瘤小鼠模型;将移植瘤小鼠分为给予百令胶囊治疗的干预组以及不进行药物治疗的模型组。治疗后30天时,测定移植瘤组织中增殖活力指标、增殖基因及侵袭基因的表达量。结果:治疗后30天时,干预组小鼠移植瘤病灶内PCNA、Ki-67的mRNA表达量及蛋白表达量均显著低于模型组,移植瘤病灶内Notch-1、Bmi-1、MIF、Rap2a、NGAL、SIRT1的mRNA表达量显著低于模型组,PAQR3、LATS1、E-cadherin、ZO-1、TES的mRNA表达量显著高于模型组。结论:虫草素能够抑制移植瘤组织中非小细胞肺癌细胞株H358的增殖及侵袭性生长。

Objective：To study the effect of cordycepin on non-small cell lung cancer cell line H358 proliferation and related gene expression.Methods：C57BL/6 mice were selected as experimental animals,and the mouse model with transplanted tumor was established after non-small cell lung cancer cell line H358 was cultured;the mice with transplanted tumor were divided into the intervention group who received Corbrin Capsule therapy and the model group who received no drug therapy.30 days after treatment,the expression of proliferation activity indexes,proliferation genes and invasion genes in transplanted tumor tissue were determined.Results：30 days after treatment,the mRNA expression and protein expression of PCNA and Ki-67 in transplanted tumor lesion of intervention group were significantly lower than those of model group,and Notch-1,Bmi-1,MIF,Rap2a,NGAL and SIRT1 mRNA expression in transplanted tumor lesion were significantly lower than those of model group while PAQR3,LATS1,E-cadherin,ZO-1 and TES mRNA expression were significantly higher than those of model group.Conclusion：Cordycepin can inhibit the proliferation and invasive growth of non-small cell lung cancer cell line H358 in transplanted tumor tissue.