子痫前期患者母体-胎盘循环超声参数与胎盘缺氧及氧化应激程度的相关性

Correlation of maternal-placental circulation ultrasound parameters with placental hypoxia and oxidative stress in patients with preeclampsia

目的:研究子痫前期患者母体-胎盘循环超声参数与胎盘缺氧及氧化应激程度的相关性。方法:选择在本院进行分娩的子痫前期患者作为研究的PE组,同期本院分娩的健康孕产妇作为研究的对照组。孕23-26周时行彩色多普勒超声测定子宫螺旋动脉的PI、RI、S/D水平;分娩后收集胎盘并测定缺氧所致细胞凋亡指标、细胞侵袭分子、氧化应激分子的表达量。结果:PE组患者子宫螺旋动脉的PI、RI、S/D水平均显著高于对照组;PE组胎盘组织中TXNIP、NLRP3、ASC、Caspase-1、Nrf-2、HO-1、NQO-1的mRNA表达量显著高于对照组且与子宫螺旋动脉PI、RI、S/D的水平呈正相关,CatL、CatB、uPA、LAMA4的mRNA表达量显著低于对照组且与子宫螺旋动脉PI、RI、S/D的水平呈负相关。结论:子痫前期患者母体-胎盘循环血流阻力增加会造成胎盘缺氧、加剧细胞凋亡及氧化应激。

Objective：To study the correlation of maternal-placental circulation ultrasound parameters with placental hypoxia and oxidative stress in patients with preeclampsia.Methods：Patients with preeclampsia who gave birth in Shandong jiaotong hospital between March 2015 and February 2017 were select as the PE group of the study,and the healthy pregnant women who gave birth in hospital during the same period were selected as the control group of the study.Color Doppler ultrasonography was done in the 23-26 weeks of pregnancy to determine the uterine spiral artery PI,RI and S/D levels;after delivery,the placenta was collected to determine the expression of hypoxia-induced apoptosis indexes,cell invasion molecules and oxidative stress molecules.Results：Uterine spiral artery PI,RI and S/D levels of PE group were significantly higher than those of control group;TXNIP,NLRP3,ASC,Caspase-1,Nrf-2,HO-1 and NQO-1 mRNA expression in placenta tissue of PE group were significantly higher than those of control group and positively correlated with uterine spiral artery PI,RI and S/D levels while CatL,CatB,uPA and LAMA4 mRNA expression were significantly lower than those of control group and negatively correlated with uterine spiral artery PI,RI and S/D levels.Conclusion：The increase of maternal-placental circulation blood resistance in patients with preeclampsia will result in the placental hypoxia,and aggravate the apoptosis and oxidative stress.