曲妥珠单抗联合紫杉醇新辅助化疗对HER-2阳性乳腺癌病灶内细胞增殖、侵袭的影响

Effect of trastuzumab combined with paclitaxel neoadjuvant chemotherapy on the cell proliferation and invasion in HER-2-positive breast cancer lesions

目的:研究曲妥珠单抗联合紫杉醇新辅助化疗对人表皮生长因子受体-2(HER-2)阳性乳腺癌病灶内细胞增殖、侵袭的影响。方法:选择2015年4月-2017年1月期间在湖北省恩施州中心医院诊断为HER-2阳性乳腺癌的患者,随机分为两组,联合组接受曲妥珠单抗联合紫杉醇化疗、对照组接受紫杉醇化疗。取手术切除的乳腺癌病灶并测定细胞增殖基因、细胞侵袭基因及血管新生分子的表达量。结果:联合组患者肿瘤病灶内USP39、EphA2、NUAK1、Gab2、Raptor、ICAM-1、HIF-1α、VEGF、ANGPLT-2、ANGPLT-4的mRNA表达量显著低于对照组,CCN5、ALEX1、ATG2B、ATG4D、E-cadherin、EBP50的mRNA表达量显著高于对照组。结论:曲妥珠单抗联合紫杉醇新辅助化疗能够抑制HER-2阳性乳腺癌病灶内细胞的增殖、侵袭并减少血管新生。

Objective：To study the effect of trastuzumab combined with paclitaxel neoadjuvant chemotherapy on the cell proliferation and invasion in human epidermal growth factor receptor-2（HER-2）-positive breast cancer lesions.Methods：Patients who were diagnosed with HER-2-positive breast cancer in the Central Hospital of Enshi Autonomous Prefecture Hubei Province between April 2015 and January 2017 were selected and randomly divided into two groups,the combined group received trastuzumab combined with paclitaxel chemotherapy,and the control group accepted paclitaxel chemotherapy.The surgically removed breast cancer lesions were collected to determine the expression of cell proliferation genes,cell invasion genes and angiogenesis molecules.Results：USP39,EphA2,NUAK1,Gab2,Raptor,ICAM-1,HIF-1α,VEGF,ANGPLT-2 and ANGPLT-4 mRNA expression in tumor lesion of combined group were significantly lower than those of control group while CCN5,ALEX1,ATG2 B,ATG4 D,E-cadherin and EBP50 mRNA expression were significantly higher than those of control group.Conclusion：Trastuzumab combined with paclitaxel neoadjuvant chemotherapy can inhibit the cell proliferation and invasion and decrease the angiogenesis in HER-2-positive breast cancer lesions.