血清中NGAL、HE4含量变化对肺癌诊断及病情进展评估的价值

Value of serum NGAL and HE4 content change for diagnosing lung cancer and evaluating disease progression

目的:研究血清中NGAL、HE4含量变化对非小细胞肺癌(NSCLC)诊断及病情进展评估的价值。方法:选择在本院诊断为NSCLC并接受手术切除的患者作为NSCLC组,选择同期体检的健康志愿者作为对照组。测定NSCLC组和对照组血清中NGAL、HE4的含量以及NSCLC组NSCLC病灶、癌旁病灶内增殖基因、凋亡基因、侵袭基因的表达量。结果:NSCLC组患者血清中NGAL、HE4的含量显著高于对照组且TNM Ⅱ期和TNM Ⅲ期NSCLC患者血清中NGAL、HE4的含量显著高于TNM Ⅰ期NSCLC患者,TNM Ⅲ期NSCLC患者血清中NGAL、HE4的含量显著高于TNM Ⅱ期NSCLC患者;NSCLC病灶中c-myc、Survivin、CatL、Vimentin、Slug、Snail的mRNA表达量显著高于癌旁病灶且与血清中NGAL、HE4的含量呈正相关,PTEN、LATS1、E-cadherin的mRNA表达量显著低于癌旁病灶且与血清中NGAL、HE4的含量呈负相关。结论:NSCLC患者血清中异常升高的NGAL、HE4能够在一定程度上评价癌细胞异常的增殖和侵袭。

Objective：To study the value of serum NGAL and HE4 content change for diagnosing non-small cell lung cancer（NSCLC）and evaluating disease progression.Methods：Patients who were diagnosed with NSCLC and underwent surgical resection in the First Affiliated Hospital of Chengdu Medical College between February 2015 and March 2017 were selected as the NSCLC group,and healthy volunteers who received physical examination over the same period were selected as control group.The NGAL and HE4 contents in serum of NSCLC group and control group as well as the expression of proliferation,apoptosis and invasion genes in NSCLC lesion of NSCLC group were detected.Results：Serum NGAL and HE4 levels of NSCLC group were significantly higher than those of control group,serum NGAL and HE4 levels of patients with TNM Ⅱ and TNM Ⅲ NSCLC were significantly higher than those of patients with TNMⅠNSCLC,and serum NGAL and HE4 levels of patients with TNM Ⅲ NSCLC were significantly higher than those of patients with TNM Ⅱ NSCLC;c-myc,Survivin,CatL,Vimentin,Slug and Snail mRNA expression in NSCLC lesion were significantly higher than those in adjacent lesion and positively correlated with serum NGAL and HE4 levels while PTEN,LATS1 and E-cadherin mRNA expression were significantly lower than those in adjacent lesion and negatively correlated with serum NGAL and HE4 levels.Conclusion：Abnormally elevated NGAL and HE4 in the serum of NSCLC patients can evaluate the proliferation and invasion of cancer cells to a certain extent.