骨肉瘤组织中自噬标志分子Beclin1的mRNA、蛋白表达量及其与病理特征的相关性

mRNA and protein expression of autophagy marker molecule Beclin1 in osteosarcoma tissue and their correlation with pathological features

目的:探讨骨肉瘤组织中自噬标志分子Beclin1的mRNA、蛋白表达量及其与病理特征的相关性。方法:2012年4月~2016年1月间在本院进行手术治疗的骨肉瘤患者60例,术中取骨肉瘤组织及瘤旁正常组织标本。采用荧光定量PCR法检测标本组织中Beclin1 mRNA及增殖、侵袭基因表达量,采用western-blot法检测标本组织中Beclin1蛋白表达量。根据骨肉瘤组织中Beclin1的mRNA、蛋白表达量中位数,将其二次分组成为Beclin1mRNA高表达组、Beclin1mRNA低表达组各30例,Beclin1蛋白高表达组、Beclin1蛋白低表达组各30例。对比骨肉瘤组织中不同Beclin1mRNA、Beclin1蛋白表达者增殖、侵袭基因表达量的差异。结果:骨肉瘤组织中Beclin1mRNA、Beclin1蛋白的表达量低于瘤旁正常组织;Beclin1mRNA低表达组骨肉瘤组织中,增殖基因KISS-1 mRNA的表达量低于Beclin1mRNA高表达组,Six1、FoxM1、RIPK4、STIM1mRNA的表达量高于Beclin1 mRNA高表达组,侵袭基因DLX1mRNA的表达量低于Beclin1 mRNA高表达组,EFEMP1、MTA1、Notch1、HES1、LEF-1mRNA的表达量高于Beclin1mRNA高表达组;Beclin1蛋白低表达组骨肉瘤组织中,增殖基因KISS-1mRNA的表达量低于Beclin1蛋白高表达组,Six1、FoxM1、RIPK4、STIM1mRNA的表达量高于Beclin1蛋白高表达组;侵袭基因DLX1 mRNA的表达量低于Beclin1蛋白高表达组,EFEMP1、MTA1、Notch1、HES1、LEF-1mRNA的表达量高于Beclin1蛋白高表达组。结论:骨肉瘤组织中自噬标志分子Beclin1的mRNA、蛋白表达量均下降,且其表达量与肿瘤细胞的增殖侵袭活性直接相关。

Objective：To discuss the mRNA and protein expression of autophagy marker molecule Beclin1 in osteosarcoma tissue and their correlation with pathological features.Methods：60 patients with osteosarcoma receiving surgical treatment in the hospital between April 2012 and January 2016 were selected,and the osteosarcoma tissue and adjacent normal tissue samples were collected during operation.Fluorescence quantitative PCR method was used to detect the expression of Beclin1 mRNA as well as proliferation and invasion gene expression in the tissue specimens,and the Beclin1 protein expression was detected by western-blot method.According to the median of Beclin1 mRNA and protein expression in osteosarcoma tissues,they were further grouped into high Beclin1 mRNA expression group（n=30）and low Beclin1 mRNA expression group（n=30）as well as high Beclin1 protein expression group（n=30）and low Beclin1 protein expression group（n=30）.The differences in proliferation and invasion gene expression in osteosarcoma tissue with different Beclin1 mRNA and Beclin1 protein expression were compared.Results：Beclin1 mRNA and Beclin1 protein expression in osteosarcoma tissue were lower than those in adjacent normal tissue;proliferation gene KISS-1 mRNA expression in low Beclin1 mRNA expression group of osteosarcoma tissue was lower than that in high Beclin1 mRNA expression group while Six1,FoxM1,RIPK4 and STIM1 mRNA expression were higher than those in high Beclin1 mRNA expression group;invasion gene DLX1 mRNA expression was lower than that in high Beclin1 mRNA expression group while EFEMP1,MTA1,Notch1,HES1 and LEF-1 mRNA expression were higher than those in high Beclin1 mRNA expression group;proliferation gene KISS-1 mRNA expression in low Beclin1 protein expression group of osteosarcoma tissue was lower than that in high Beclin1 protein expression group while Six1,FoxM1,RIPK4 and STIM1 mRNA expression were higher than those in high Beclin1 protein expression group;invasion gene DLX1 mRNA expression was lower than that in high Beclin1 protein expression group while EFEMP1,MTA1,Notch1,HES1 and LEF-1 mRNA expression were higher than those in high Beclin1 protein expression group.Conclusion：Both mRNA and protein expression of autophagy marker molecule Beclin1 decrease in osteosarcoma tissue and are directly correlated with the proliferation and invasion activity of tumor cells.