MEST对子痫前期胎盘中新生血管形成、滋养细胞侵袭的影响

Effect of MEST on the angiogenesis and trophoblast cell invasion in the preeclampsia placenta

目的:研究中胚层特异性转录基因(MEST)对子痫前期胎盘中新生血管形成、滋养细胞侵袭的影响。方法:选择2014年6月~2017年2月三院、四院诊断为子痫前期并分娩的产妇作为PE组,选择同期在两院分娩的健康产妇作为对照组。分娩后,采集胎盘组织并采用荧光定量PCR法测定MEST的mRNA表达量,采用酶联免疫吸附法测定血管新生分子、细胞侵袭分子的蛋白含量。结果:PE组胎盘组织中MEST、Notch1、DLL4、VEGF、Rho、ROCK、LAMA4、Notch2、Notch3的蛋白含量显著低于对照组,DNC、sFlt-1、sEng的蛋白含量显著高于对照组;与MEST高表达的PE胎盘组织比较,MEST低表达的PE胎盘组织中Notch1、DLL4、VEGF、Rho、ROCK、LAMA4、Notch2、Notch3的蛋白含量降低,DNC、sFlt-1、sEng的蛋白含量升高。结论:子痫前期胎盘中低表达的MEST能够抑制新生血管的形成以及滋养细胞的侵袭。

Objective:To study the effect of mesoderm-specific transcript(MEST)on the angiogenesis and trophoblast cell invasion in the preeclampsia placenta.Methods:The puerperae who were diagnosed with preeclampsia and gave birth in our hospital between June 2014 and February 2017 were selected as the PE group,and the healthy puerperae who gave birth in our hospital during the same period were selected as the control group.After delivery,the placental tissue was collected,fluorescence quantitative PCR method was used to determine MEST mRNA expression,and enzyme-linked immunosorbent assay was used to determine the protein levels of angiogenesis molecules and cell invasion molecules.Results:MEST,Notch1,DLL4,VEGF,Rho,ROCK,LAMA4,Notch2 and Notch3 protein levels in placental tissue of PE group were obviously lower than those of control group whereas DNC,sFlt-1 and sEng protein levels were obviously higher than those of control group;Notch1,DLL4,VEGF,Rho,ROCK,LAMA4,Notch2 and Notch3 protein levels in PE placenta tissue with lower MEST expression were greatly lower than those in PE placenta tissue with higher MEST expression whereas DNC,sFlt-1 and sEng protein levels were greatly higher than those in PE placenta tissue with higher MEST expression.Conclusions:The low expression of MEST in preeclampsia placenta can inhibit the angiogenesis and trophoblast cell invasion.