甲状腺恶性结节超声弹性成像应变率与癌基因、血管新生基因表达的相关性

The correlation of the ultrasonic elastography strain rate of malignant thyroid nodules with the expression of oncogenes and angiogenesis genes

目的:研究甲状腺恶性结节超声弹性成像应变率与癌基因、血管新生基因表达的相关性。方法:选择2015年3月~2017年2月期间在我院接受超声检查的甲状腺结节患者,根据细针穿刺活检组织结果判断组织良恶性;进行超声弹性成像并测定应变率比值,进行荧光定量PCR反应并测定癌基因、血管新生基因的mRNA表达量。结果:甲状腺恶性结节的应变率比值显著低于甲状腺良性结节;甲状腺恶性结节中CCNG、RASSF1A的mRNA表达量显著低于甲状腺良性结节,FF3、TPX2、WIP1、Ang2、Tie2、VEGF、VEGFR1、c-met、Survivin的mRNA表达量显著高于甲状腺良性结节;低应变率比值的甲状腺恶性结节中CCNG、RASSF1A的mRNA表达量显著低于高应变率比值的甲状腺恶性结节,TFF3、TPX2、WIP1、Ang2、Tie2、VEGF、VEGFR1、c-met、Survivin的mRNA表达量高于高应变率比值的甲状腺恶性结节。结论:甲状腺恶性结节超声弹性成像应变率比值的升高与癌基因及血管新生基因表达的变化密切相关。

Objective:To study the correlation of the ultrasonic elastography strain rate of malignant thyroid nodules with the expression of oncogenes and angiogenesis genes.Methods:Patients with thyroid nodules who underwent ultrasonography in this hospital between March 2015 and February 2017 were selected,and the tissue properties were judged according to the results of fine needle aspiration biopsy;ultrasonic elastography was done to measure the strain rate ratio,and the fluorescence quantitative PCR reaction was performed to determine the mRNA expression of oncogenes and angiogenesis genes.Results:The strain rate ratio of malignant thyroid nodules was greatly lower than that of benign thyroid nodules;CCNG and RASSF1A mRNA expression in malignant thyroid nodules were greatly lower than those in benign thyroid nodules whereas FF3,TPX2,WIP1,Ang2,Tie2,VEGF,VEGFR1,c-met and Survivin mRNA expression were greatly higher than those in benign thyroid nodules;CCNG and RASSF1A mRNA expression in malignant thyroid nodules with low strain rate ratio were greatly lower than those in malignant thyroid nodules with high strain rate ratio whereas FF3,TPX2,WIP1,Ang2,Tie2,VEGF,VEGFR1,c-met and Survivin mRNA expression were higher than those in malignant thyroid nodules with high strain rate ratio.Conclusion:The increase in ultrasonic elastography strain rate ratio of malignant thyroid nodules is closely related to the changes in the expression of oncogenes and angiogenesis genes.