神经特异型基因c-src产物pp^(60c-src(+))在突触形成中的表达

Expression of neuron-specific c-src gene product pp^(60c-src(+)) in synaptogenesis

目的 :为了探索神经特异型基因c src产物 pp60c src(+ ) 在突触形成中的作用机制。方法 :用免疫细胞化学方法检测pp60c src(+ ) 在初代培养鸡胚脊神经节细胞体、突起和生长端内的分布特征。结果 :培养 2 4小时的生长端丝状伪足不断运动改变生长端的形态 ,pp60c src(+ ) 的免疫反应活性分布在神经细胞的核周体、神经突起、生长端部分领域和丝状伪足 ;培养 4 8小时的生长端接近靶细胞 ,丝状伪足的活泼性和运动性明显减弱以至消失 ,在伸长的突起上有pp60c src(+ ) 免疫反应活性较强的串珠样膨体 ,在生长端体部的部分区域和丝状伪足免疫反应活性明显减弱。结论 :( 1 )在生长端向突触前部分化的过程中 ,pp60c src(+ ) 免疫活性曾发生一过性的减低或消失 ,它具有时空特异性 ;( 2 )pp60c src(+ ) 对突触发育具有重要的调控作用。

Objective:To investigate the role of pp 60c-src(+) ,the product of neuron-specific c-src gene,in synaptogenesis.Methods:The distribution feature of pp 60c-src(+) in the neuronal cell bodies,processes and growth cones were detected in the primarily cultured chicken spinal ganglion cells by immunocytochemistry (double immunofluorescent staining and PAP technique).Results:Filopodia in the growth cones,which had been cultured for 24 h,moved unceasingly,and the morphological characteristics of growth cones changed.Immunoactivity of pp 60c-src(+) distributed around perikaryon,nerve processes,parts of growth cones and filopodia.Growth cones cultured for 48 h were close to target cells.Activity and movement of filopodia significantly decreased and even disappeared.In the stretching processes,there were varicosities where the immunoactivity of pp 60c-src(+) was strong,while in some parts of growth cone bodies and filopodia,the immunoactivity was weaker.Conclusion:(1) In the process when growth cones differentiated to the anterior part of synapsis,the immunoactivity of pp 60c-src(+) transiently decreased or disappeared,which had the time-space specificity.(2) pp 60c-src(+) had the important control effect of synaptogenesis.