王浆酸的高效液相色谱分析及抑菌活性研究

Determination of 10-HDA by HPLC and its studies on bacteriostatic activity

采用高效液相色谱法对蓖麻油等为原料合成的王浆酸(10-HDA)进行了定量分析,色谱条件为C18型色谱柱,流动相为:无水甲醇/水/0.01 mol/L盐酸=55/35/10(体积比),流速为0.6 m L/min,波长为212 nm,柱温为40℃,进样体积为5μL。建立了一种实验室内快速准确分析王浆酸含量的简便检测方法。同时,对王浆酸的抑菌活性进行了室内研究,其结果与蜂王浆中分离出来的王浆酸效果相当。

Adopting HPLC to analyze the quantity of 10-hydroxy-2-decenoic acid( 10-HDA) synthesized by castor oil and other raw materials. The chromatographic conditions: C18 column,CH_3OH/H_2O/0. 01 mol/L HCl( 55/35/10,volume ratio) were used as the mobile phase,the flow rate was 0. 6 m L/min,the UV detection wavelength was fixed at 212 nm,column temperature was 40 ℃,and injection volume was 5 μL. Herein,a fast and accurate detection method of 10-HDA was established by HPLC. At the same time,the bacteriostatic activity of 10-HDA was studied in the laboratory,and it was found that it hold equivalent result compared with 10-HDA separated from the royal jelly.