胎肾细胞悬液对去卵巢大鼠骨组织形态计量学参数的影响

Effect of Fetal Renal Cell Suspension on Bone Histomorphometry in Ovariectomized Rats

目的:观察胎肾细胞悬液对去卵巢大鼠骨组织形态计量学参数的影响,探讨胎肾对大鼠去卵巢所致骨质疏松症的作用。方法:48只SD雌性大鼠,随机分为5组,即假手术组、模型组、雌激素组、胎肾活细胞组和胎肾死细胞组。假手术组仅手术切除腹腔少量脂肪,其余各组行去卵巢手术造模。术后12周,假手术组及模型组使用生理盐水干预,雌激素组使用尼尔雌醇混悬液干预,胎肾活细胞组使用胎肾活细胞悬液干预,胎肾死细胞组使用胎肾死细胞悬液干预。各组连续用药干预4周后,取血清行血清雌激素水平检测,取股骨远端1/3做骨病理切片,行骨组织形态计量学观测。结果:与假手术组比较,模型组雌激素水平显著降低,差异有统计学意义(P<0.01)。骨小梁排列稀疏、无序,骨小梁间隙显著增大,差异有统计学意义(P<0.01)。骨小梁密度、骨小梁厚度、骨小梁面积百分比均显著降低,差异有统计学意义(P<0.01)。与模型组比较,雌激素组、胎肾细胞各组的雌激素水平均显著升高,差异有统计学意义(P<0.01)。骨小梁排列较紧密、排列较整齐、交织成网,骨小梁间隙显著缩小,差异有统计学意义(P<0.01)。骨小梁密度、骨小梁厚度、骨小梁面积百分比均显著增加,差异有统计学意义(P<0.01)。与雌激素组比较,胎肾细胞各组雌激素水平差异无统计学意义(P>0.05),但胎肾死细胞组骨小梁间隙显著缩小且骨小梁密度显著增加,差异有统计学意义(P<0.01)。与胎肾活细胞组比较,胎肾死细胞组骨小梁间隙显著缩小、骨小梁密度显著增加,差异有统计学意义(P<0.05)。结论:胎肾细胞各组对去卵巢大鼠骨量的增加、骨小梁结构及其连接性的改善、血清雌二醇水平的提高有明显作用,说明胎肾有治疗大鼠去卵巢所致骨质疏松症的作用。

Objective： To observe the effect of fetal renal cell suspension（FRCS）on bone histomorphometry parameters in ovariectomized rats,and to investigate the effect of fetal renal on osteoporosis in ovariectomized rats. Methods： All 48 female SD rats were randomly divided into five groups,including sham group, model group, estrogen group, fetal renal living cells group（FRLC group）and fetal renal death cell group（FRDC group）. In sham group,only a small amount of fat was removed from the abdominal cavity,and all the other groups were underwent ovarian surgery. Twelve weeks after operation, the sham group and model group were treated with physiological saline, and estrogen group was treated with Neal estradiol suspension. The FRLC group was treated with fetal renal living cell suspension,and the FRDC group was treated with re-tal renal dead cell suspension. After continuous intervention for 4 weeks, serum levels of estrogen were detected, and the structure of the 1/3 of the distal femur was observed and detected with pathological image analysis system. Results：Compared with sham group, the estrogen level of model group was significantly lower （P 〈 0.01 ）, trabecular sparse, disor-der, trabecular gap significantly increased（P〈0.01 ） ; trabecular bone density, trabecular thickness, percentage of trabecular bone area decreased significantly（P〈0.01）. Compared with model group,in fetal renal cell groups the level of estrogen were significantly increased（P〈0.01）, trabecular arranged closely and neatly, woven into a net, trabecular gap was significantly reduced（P〈0. 01）;trabecular bone density, trabecular thickness and the percentage of trabecular bone area increased significantly（P〈0.01）. Compared with estrogen group,the level of estrogen were not significantly difference in fetal renal cell groups（P〉0.05） ,but in FRDC group trabecular gap was significantly reduced and trabecular bone density was significantly increased（PM0.01）. Compared with FRLC group, trabecular bone density in FRDC group increased significantly（P〈 0.01 ）, and trabecular gap significantly decreased （P〈 0.0 5）. Conclusion ： The fetal renal cell groups have obvious effect on increasing bone mass,improving trabecular bone structure and connectivity,increasing serum estrogen level of ovariectomized osteoporosis rats. This study suggests that fetal renal cell suspension could effectively treat the osteopo- rosis induced by ovariectomy in rats.