Kruppel样转录因子9在上皮性卵巢癌组织中的表达及临床意义

Expression and clinical significance of Kruppel-like factor-9 in patients with epithelial ovarian carcinoma

目的探讨Kruppel样转录因子9(KLF9)在上皮性卵巢癌中的表达及其临床意义。方法搜集63例具有完整临床病理资料及5年以上随访资料的石蜡包埋上皮性卵巢癌组织及正常卵巢上皮组织,采用荧光定量PCR技术检测KLF9 m RNA在上皮性卵巢癌及正常卵巢组织中的表达水平,应用免疫组化法分析KLF9在上皮性卵巢癌组织和正常卵巢组织的表达情况,分析KLF9表达水平与临床病理参数和患者预后的关系。结果 63例癌组织中KLF9 m RNA的表达值为(0.597±0.332)显著低于正常卵巢组织中KLF9 m RNA的表达(1.354±0.471),差异有统计学意义(P<0.01)。上皮性卵巢癌组织中KLF9高表达15例(23.8%),低表达48例(76.2%);在正常卵巢组织中KLF9高表达18例(60.0%),低表达12例(40.0%),差异有统计学意义(P<0.01)。癌组织中KLF9低表达与上皮性卵巢癌患者的临床分期、淋巴结转移、腹腔积液明显相关(P<0.05)。KLF9 m RNA高表达组中位生存时间46.8个月(95%CI:26~83个月)显著高于低表达组32.6个月(95%CI:12~74个月),差异有统计学意义(P<0.01)。肿瘤组织中KLF9阳性细胞高表达组的中位生存时间为50.2个月(95%CI:32~84个月)明显高于KLF9低表达组31.1个月(95%CI:12~79个月),差异有统计学意义(P<0.01)。Cox多重回归分析表明KLF9 m RNA和KLF9阳性细胞低表达是影响上皮性卵巢癌预后不良的独立指标。结论 KLF9在上皮性卵巢癌组织中表达下调,与上皮性卵巢癌患者预后不良相关。

Objective To explore the expression and clinical significance of Kruppel-like factor-9 （KLF9） in patients with epithelial ovarian carcinoma.Methods Paraffin-embedded epithelial ovarian carcinoma tissue samples and normal ovarian epithelial tissue samples of 63 patients with complete clinicopathological data and follow-up data more than five years were collected.Fluorescent quantitative PCR was used to detect the expression levels of KLF9 mRNA in epithelial ovarian carcinoma tissue and normal ovarian tissue.Immunohistochemical method was used to analyze the expressions of KLF9 in epithelial ovarian carcinoma tissue and normal ovarian tissue.The relationships between KLF9 expression level and clinicopathological parameters,prognosis were analyzed.Results The expression level of KLF9 mRNA in epithelial ovarian carcinoma tissue was （0.597 ± 0.332）,which was statistically significantly lower than that in normal ovarian tissue （1.354 ± 0.471） （P〈0.01）.The expression levels of KLF9 in 15 patients （23.8%） with epithelial ovarian carcinoma were high,and the expression levels of KLF9 in 48 patients （76.2%） with epithelial ovarian carcinoma were low.Among the women with normal ovarian tissue,KLF9 expression levels were high in 18 women （60.0%）,and KLF9 expression levels were low in 12 women （40.0%）,there was statistically significant difference （P〈0.01）.The low expression of KLF9 in epithelial ovarian carcinoma was significantly correlated with clinical stages,lymph node metastasis,and ascetic fluid （P〈0.05）.The median survival time in KLF9 mRNA high expression group was 46.8 months （95% CI：26-83 months）,which was statistically significantly higher than that in KLF9 mRNA low expression group （32.6 months） （95% CI：12-74 months） （P〈0.01）.Among the patients with epithelial ovarian carcinoma,the median survival time of KLF9 high expression group was 50.2 months （95% CI：32-84 months）,which was statistically significantly higher than that in KLF9 mRNA low expression group （31.1 months） （95% CI：12-79 months） （P〈0.01）.Cox multiple regression analysis showed that low expressions of KLF9 mRNA and KLF9 positive cells were independent influencing indexes of adverse prognosis of epithelial ovarian carcinoma.Conclusion The expression of KLF9 is down-regulated in epithelial ovarian carcinoma,which is correlated with adverse prognosis of patients with epithelial ovarian carcinoma.