依折麦布与考来替泊交替给药对于高脂血症大鼠脂代谢的调节作用

Alternate dosing of ezetimibe and colestipol on regulating lipid metabolism in hyperlipidemia rats

目的:探讨依折麦布和考来替泊交替给药对于高脂血症大鼠的脂代谢调节作用。方法:选用清洁级的SD大鼠50只,雌雄各半,通过高脂饲料饲养造模,造模成功后随机分组,设置正常组、模型组、实验组(依折麦布组、考来替泊组、依折麦布+考来替泊组),实验组每日灌胃给药,正常组和模型组给予生理盐水,连续给药8周,检测血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDLC)含量以及乙酰辅酶A羧化酶(ACC)、脂肪酸合酶(FAS)、3-羟基-3-甲基戊酰辅酶A还原酶(HMGR)、肝脏肉毒碱棕榈酰转移酶I(CPT-I)的mRNA的表达。结果:实验组大鼠血清TC、TG、HDL-C、LDL-C含量以及ACC、FAS、HMGR、CPT-I的mRNA表达相比模型组均有降低且交替用药组较单一用药组含量显著降低(P<0.05)。结论:依折麦布和考来替泊交替给药对于高脂血症大鼠脂代谢的调节优于单一用药,但是对人体的作用还需要临床验证。

AIM： To explore alternate dosing of czetimibe and colestipol on regulating for lipid metabolism in hyperlipidemia rats. METHODS：Fifty rats of clean grade, half male and female were modeling through feeding high fat diet; the rats were randomly divided into normal group, model group, and experimental groups （ including ezetimibe group, colestipol group, ezetimibe ＋ colestipol group） ; experimental groups received gavage administration, while the normal group and model group were given normal saline for 8 weeks; TG, TC, HDL-C, LDL-C and ACC, FAS, HMGR, CPT-I expression of mRNA in serum were thereafter detec-ted. RESULTS ：The levels of TC, TG, LDL-C and AAC, FAS, HMGR mRNA in serum were decreased, and levels of ezetimibe ＋ colestipol group were significantly lower than those of single drug group （ P 〈 0.05 ）. CONCLUSION ： Alternate dosing of ezetimibe and colestipol was better than single medication in regulating lipid metabolism in hyperlipidemia rats, but the effect on human body still needs further clinical validation.