儿童川崎病患者血浆和肽素,IL-17A,TNF-α检测与冠脉病变的相关性研究

Study on the Relationship between Peptides and Interleukin-17A,Tumor Necrosis Factorαin Children Kawasaki Coronary Artery Disease

目的探讨血浆和肽素,IL-17A及TNF-α在儿童川崎病冠脉病变中的诊断意义。方法选取经临床确诊的川崎病冠脉病变患儿60例(男性32例,女性28例,平均年龄2.5岁),同时选取健康体检儿童40例(男性23例,女性17例,平均年龄3岁)为对照组。采用酶联免疫吸附试验(ELISA)法检测各组血浆和肽素、白介素17A(IL-17A)、肿瘤坏死因子α(TNF-α)的含量。结果 (1)川崎病冠脉病变组血浆中的和肽素(21.09±2.72pmol/L),IL-17A(21.20±2.35pg/ml),TNF-α(50.84±5.81pg/ml)含量均高于对照组(18.45±2.96pmol/L,18.80±2.20pg/ml,45.24±6.15pg/ml),t=6.13,8.586,7.278,均P<0.01。(2)受试者工作曲线(receiver operating characteristic curve,ROC)显示,和肽素预测川崎病冠脉病变敏感度为88.2%,特异度为75.0%;IL-17A敏感度为85.3%,特异度为79.2%;TNF-α敏感度为88.2%,特异度为73.6%。结论川崎病冠脉病变患儿急性期血浆和肽素,IL-17A和TNF-α水平均升高,三者联合可以预测川崎病患儿冠脉病变的发生。

Objective To investigate the diagnostic value of Peptides and Interleukin-17A,Tumor necrosis factorαin children kawasaki coronary artery disease.Methods 60 patients,made a definite diagnosis as kawasaki coronary artery disease（32 cases of male,28 cases of female,average age 2.5 years）,40 healthy subject were enrolled in the study（23 cases of male,fe-male 17 cases,with an average age of 3 years of age）.The plasma levels of Peptides,Interleukinin 17A（IL-17A）and Tumor necrosis factorα（TNF-α）in each group was detected by ELISA.Results ①The plasma levels of Peptides,Interleukinin 17A （IL-17A）and Tumor necrosis factorα（TNF-α）was significantly higher than healthy controls,respectively（21.09±2.72） vs（18.45±2.96）pmol/L,t=6.13,P〈0.01,（21.20±2.35）vs（18.80±2.20）pg/ml,t=8.586,P〈0.01;（50.84±5.81） vs（45.24±6.15）pg/ml,t=7.278,P〈0.01.②Results of ROC curves showed,the sensitivity of Peptides in kawasaki coro-nary artery disease was 88.2%,the specificity was 75.0% and the sensitivity of IL-17A was 85.3%,the specificity was 79.2%,the sensitivity of TNF-αwas 88.2%,the specificity was 73.6%.Conclusion The plasma levels of Peptides,IL-17A and TNF-αin acute phase of kawasaki coronary artery disease were all increased.Combined detections of peptide,IL-17A and TNF-αmay predict the incidence of coronary artery disease in children with kawasaki disease.