整合素αvβ3、趋化因子受体4和7在头颈部鳞状细胞癌中的表达及其与淋巴结转移的关系

Expression of integrin αvβ3, CXC chemokine receptor 4 and CXC chemokine receptor 7 and their relationship with lymph node metastasis in squamous cell carcinoma of head and neck

目的 探讨整合素αvβ3、趋化因子受体（CXC chemokine receptor,CXCR）4、CXCR7在头颈部鳞状细胞癌（squamous cell carcinoma of head and neck,SCCHN）及颈淋巴结组织中的表达及其在淋巴结转移中的作用.方法 收集2010年1月至2015年1月郑州大学附属肿瘤医院头颈甲状腺外科92例SCCHN,应用免疫组织化学SABC法检测SCCHN、淋巴结转移灶、正常淋巴结以及正常黏膜组织中整合素αvβ3、CXCR4和CXCR7的表达情况.结果 整合素αvβ3、CXCR4和CXCR7主要表达于SCCHN的胞质和胞膜,三者在SCCHN原发灶中阳性表达率分别为75％（69/92）、82％（75/92）和76％（70/92）,在淋巴结转移灶中阳性表达率分别为81％（75/92）、76％ （70/92）和65％（60/92）.整合素αvβ3、CXCR4在SCCHN原发灶（r=0.813,P＜0.05）和淋巴结转移中（r=0.541,P＜0.05）的表达呈正相关;整合素αvβ3及CXCR7在SCCHN原发灶（r=0.683,P＜0.05）和淋巴结转移中（r=0.708,P＜0.05）的表达呈正相关;CXCR4、CXCR7在SCCHN原发灶（r=0.644,P＜0.05）和淋巴结转移中（r=0.707,P＜0.05）的表达呈正相关.整合素αvβ3、CXCR4和CXCR7表达的强弱与肿瘤大小（P=0.040、0.001、0.009）、淋巴结转移（P=0.001、0.000、0.000）、有无周围组织侵犯（P=0.046、0.002、0.001）显著相关,与患者年龄（P=0.097、0.274、0.162）、性别（P=0.103、0.309、0.187）无关.结论 整合素αvβ3、CXCR4、CXCR7在SCCHN原发灶组织及转移淋巴结中呈高表达,并与淋巴转移显著相关,三者可能共同促进SCCHN的淋巴结转移.

Objective To investigate the expression of integrin αvβ3,CXC chemokine receptor （CXCR）4 and CXCR7 and their relationship with lymph node metastasis in squamous cell carcinoma of head and neck （SCCHN）.Methods The expression of integrin αvβ3,CXCR4 and CXCR7 was detected by immunohistochemistry SABC in 92 cases of primary SCCHN,metastatic lymph node,normal oral mucosa tissues and normal lymph nodes.Results The positive rate of the expression of integrin αvβ3,CXCR4 and CXCR7 was 75% （69/92）,81%（75/92） and 76%（70/92）,respectively in primary SCCHN,and was 82% （75/92）,76%（70/92） and 65%（60/92）,respectively in metastatic lymph node.The expression of integrin αvβ 3 and CXCR4 in primary SCCHN （r=0.813,P〈0.05） and lymph node metastasis （r=0.541,P〈0.05） was positively correlated.Integrin αvβ3 and CXCR7 expression in primary SCCHN （r=0.683,P〈0.05） and lymph node metastasis （r=0.708,P〈0.05） was positively correlated.CXCR4 and CXCR7 expression in primary SCCHN （r=0.644,P〈0.05） and lymph node metastasis （r=0.707,P〈0.05） had a positive correlation.The expression level was associated with tumor size （P=0.040,0.001,0.009）,lymph node metastasis （P=0.001,0.000,0.000） and surrounding tissue invasion （P=0.046,0.002,0.001）,but not related to age （P=0.097,0.274,0.162）,gender （P=0.103,0.309,0.187）.Conclusions The overexpression of integrin αvβ3,CXCR4 and CXCR7 in primary head and neck squamous carcinoma and metastatic lymph nodes was related to lymph node metastasis.The co-expression of integrin αvβ3,CXCR4 and CXCR7 may play a synergistic role in lymphatic metastasis of SCCHN.