山楂叶四种不同提取部位指纹图谱和总黄酮含量的比较研究

COMPARATIVE STUDY ON FINGER-PRINT AND TOTAL FLAVONOIDS CONTENT IN FOUR DIFFERENT EXTRACTION PARTS OF HAWTHORN LEAVES

目的:通过建立高效液相色谱法(HPLC)指纹图谱和测定总黄酮含量,比较分析山楂叶四种不同提取部位:山楂叶50%乙醇提取部位(A)、中国药典收录的山楂叶过大孔树脂后醇提取物(B)、山楂叶乙酸乙酯部位(C)、山楂叶正丁醇部位(D)。方法:采用HPLC法建立山楂叶指纹图谱,采用Diamonsil C18(4.6mm×250mm,5μm)色谱柱,乙腈-0.1%冰醋酸-四氢呋喃为流动相梯度洗脱,检测波长320nm,流速0.9ml/min,柱温30℃。总黄酮含量测定采用紫外分光光度法。结果:山楂叶四种提取物指纹图谱共有峰的种类和数量存在差异,C与其它三种提取物的差别最大;提取物B的总黄酮含量最高。结论:本研究可为山楂叶提取物的进一步开发利用奠定基础,也能为研究山楂叶不同提取部位药效学差异提供一定的参考。

Objective： To comparatively analyze 4 different extraction parts（A.50% ethanol extract from hawthorn leaf,B.alcohol extract of hawthorn leaf after macroporous resin,C.ethyl acetate part of hawthorn leaf,D.n-butanol part of hawthorn leaf）. of hawthorn leaves by establishing HPLC finger-print and detecting total flavonoids content. Methods：The fingerprint of hawthorn leaves was established by HPLC method using Diamonsil C18（4.6 mm×250 mm, 5 μm） chromatographic column, acetonitrile-0.1% acetic acid-tetrahydrofuran as mobile phase with gradient elution, detection wavelength 320 nm, flow rate of 0.9 ml/min, and the column temperature was 30℃. The total flavonoids content was detected by ultraviolet spectrophotometry. Results： The common peaks of 4 extraction parts of hawthorn leaves in types and numbers had differences; The differences between extract C and the other three extracts was the largest. The total flavonoids content in extract B was the highest. Conclusions： This study can lay foundation for further development and utilization of hawthorn leaves extracts, and also can provide references for studying pharmacodynamic differences of different extraction parts of hawthorn leaves extracts.