转录因子MoOaf22对稻瘟病菌营养生长、细胞壁完整性和胁迫应答的调控

Regulation of transcription factor MoOaf22 in vegetative growth,cell wall integrity and stress response in Magnaporthe oryzae

为了研究油酸激活型转录因子MoOaf22编码基因MoOAF22(MGG_03413)在稻瘟病菌生长发育及致病过程中的功能,利用基因敲除方法将其进行了缺失突变并对突变体进行了一系列的生物学表型分析。结果表明该基因缺失突变体在完全培养基(CM)上营养生长加快,但在基本培养基(MM)上生长变慢,同时该突变体原生质体释放速度变慢,对荧光增白剂(CFW)和刚果红(Congo Red)耐受性增强,对十二烷基磺酸钠(SDS)、氯化钠(Na Cl)和山梨醇(Sorbitol)敏感,但该突变体在产孢量、附着胞形成及致病性等方面较野生型没有明显变化。说明MoOaf22在稻瘟病菌的营养生长、细胞壁完整性和对外界的胁迫应答过程中具有重要的调控作用。

To study the function of oleate-activated transcription factor MoOaf22 coding gene （ MGG_03413） on growth and pathogenicity,the deletion mutant was identified using the knock out method in the rice blast fungus Magnaporthe oryzae. The results indicated that the deletion mutant showed increased growth and reduced growth in complete medium （CM） and minimal medium （MM）, respectively. Meanwhile,the rate of protoplast release of the mutant was slo-wer than that of the wild type,and the mutant was more resistant to calcofluor white （ CFW） and congo red,in contrast, the mutant was more sensitive to sodium dodecyl sulfate （ SDS）,sodium chloride （NaCl） and sorbitol. However,the mu-tant showed no difference in conidiation,appressorium formation and pathogenicity compared with wild type. In summary,MoOaf22 is involved in vegetative growth, cell wall integrity and response to stress of M. oryzae.