CC类趋化因子22和叉头状翅膀样转录因子3在胆管癌组织中的表达及意义

Expression and significance of CCL22 and FOXP3 in cholangiocarcinoma

目的探讨CC类趋化因22(C-C class chemokines 22,CCL22)和叉头状翅膀样转录因子3(forkhead/winged helix transcription factor 3,FOXP3)在胆管癌组织中的表达及其临床意义。方法采用免疫组织化学法检测70例胆管癌组织和15例非肿瘤胆道上皮组织中CCL22、FOXP3的表达情况,分析其与胆管癌临床和预后的关系。结果 70例胆管癌、15例非肿瘤胆道上皮组织中CCL22和FOXP3阳性表达率分别为62.4%(44/70)、20.0%(3/15)及71.4%(50/70)、13.3%(2/15)。CCL22和FOXP3蛋白在胆管癌组织中的表达水平均明显高于非肿瘤胆道上皮组织(P<0.01)。有淋巴结转移者和TNM分期Ⅲ+Ⅳ期者较无淋巴结转移者和TNM分期Ⅰ+Ⅱ期者CCL22和FOXP3的表达阳性率高,差异有统计学意义(P<0.05)。CCL22与FOXP3蛋白表达呈明显正相关(P<0.01)。CCL22和FOXP3蛋白阴性表达者生存时间明显长于阳性表达者(P<0.01)。结论 CCL22和FOXP3可能对胆管癌发展及预后产生重要影响。

Objective To investigate the expression of C-C class chemokines 22(CCL22)and the specific marker of regulatory T cells(Tregs)forkhead/winged helix transcription factor 3(FOXP3)and to analyze the correlation and the relationship with clinicopathological factors and prognosis in order to explore the effect of Tregs on tumor immunity in cholangiocarcinoma.Methods The expression of CCL22 and FOXP3 in 70 cases of cholangiocarcinoma and 15 cases of paracancer were detected by immunohistochemical staining.Results The positive expression rates of CCL22 and FOXP3 in 70 cases of cholangiocarcinoma,15 cases of non-tumor bile duct epithelium were 62.4%,20.0% and 71.4%,13.3%,respectively.There was a significant difference among different groups(P<0.01).The positive rates of CCL22 and FOXP3 were significantly higher in the stageⅢ+Ⅳ and lymph nodes metastasis patients than those in stageⅠ+ Ⅱ and no lymph nodes metastasis patients(P<0.05).The expression of CCL22 was positively correlated with that of FOXP3(P <0.01). Moreover survival time of negative expression in CCL22 and FOXP3 patients was longer than that of positive expression in CCL22 and FOXP3 patients(P<0.01).Conclusion CCL22 and FOXP3 may have great influence on development and prognosis of cholangiocarcinoma.