番茄溃疡病病原菌遗传多样性分析

Analysis of genetic diversity in tomato canker pathogenic bacteria

为了解不同来源地菌株的亲缘关系,进而探寻不同菌株间的遗传差异,试验运用分子技术对采自不同地区的43株番茄溃疡病菌菌株进行DNA遗传系统发育分析,通过使用Blast程序进行相似性比较,结果表明:43个菌株为番茄溃疡病菌,其相似度达到99%以上。分析采集来源于同一地域、同一年份,特别是同一品种的溃疡菌菌株,均聚在一个进化分支上。

In order to understand the relationship between strains from different origin, the genetic differences were explored between different strains.This study focus on using molecular techniques to analyze the DNA genetic phylogenetic of tomato canker pathogenic bacteria which collected from different areas, comparing the similarity of 43 strains by Blast method. The results showed that the 43 strains from different origin all were tomato canker pathogenic bacteria and the similarity was more than 99%. And the strains collected from the same area and year, especially the same tomato variety were almost clustered on the one branch.