金叶榆组培快繁技术体系的建立

Rapid propagation technology system of Ulmus pumila tissue culture

金叶榆(Ulmus pumila),榆科榆属,又称美人榆,有很强的生长能力且枝繁叶茂,在园林造景方面有很大景观价值。文章以金叶榆的茎段、腋芽、叶片为外植体,采用不同浓度激素处理,对外植体的消毒方法和诱导培养进行筛选和探索,更快地创建金叶榆组织培养快速繁殖技术体系。结果表明:最佳培养基是MS培养基(附加蔗糖20 g/m L),0.1%的Hg Cl2为最佳消毒剂,消毒时间为6 min;不同外植体在各种激素配比中诱导率有很大差异,其中茎段的愈伤组织在0.5 mg/L 6-BA+2.00 mg/L NAA条件下,诱导率相对较高;腋芽发生不定芽增殖数最多是在1.0 mg/L 6-BA+0.25 mg/L NAA条件下;叶片在4.0 mg/L 6-BA+1.00 mg/L NAA组合中,诱导率最高,该研究成果优化了金叶榆组织培养快繁体系的生长条件。

Ulmus pumila,also known as beauty elm,could be trimmed to various shape for its much branches and full crown, and it played an important role on landscape. In order to establish the rapid propagation system of Ulmus pumila,using the stem, axillary buds and leaves of Ulmus pumila as explants, the effects of different disinfection methods and induction culture on the rapid propagation of Ulmus pumila were studied. The results showed that the optimum medium was MS medium with sucrose 20 g/m L, the best disinfectant was 0.1% Hg Cl2. The disinfection time was 6 min. The induction rate of different explants was different in different hormones. Under the condition of 0.5 mg/L 6-BA＋2.00 mg/L NAA, the callus induction rate of stem section was relatively high. The largest number of axillary buds adventitious bud proliferation was 1.0 mg/L 6-BA ＋0.25 mg/L NAA. In the 4.0 mg/L 6-BA ＋1.00 mg/L NAA combination, blades induction rate were the highest. The results can provide reference for the optimization of the tissue culture and rapid propagation of Ulmus pumila.