电镜半薄切片技术的几点改进

Improvement of Semi-thin Slices of Electron Microscopy Methods

为了探讨半薄切片技术的改进方法,和常规的半薄切片技术相比,该文实验在染液、染色时间、防脱片和捞片工具方面进行了改进。使用改进后的半薄切片技术,得到的组织细胞结构完整,染色清晰,层次分明。实践表明,改进技术更适用于透射电子显微镜超薄切片的准确定位,是一种简便易行、效果良好的方法。

Compared with the conventional semi-thin slicing technique, the improved method of semi-thin slicing technology has been improved in dyeing, dyeing time, anti-disc and chip tools. Using the improved semi-thin slicing technique, the resulting tissue structure is complete, the staining is clear and the level is clear. Practice show,s that the improved technology is more suitable for the accurate positioning of the semi-thin sections of the transmission electron microscope, which is a simple and effective method.