液相悬浮芯片技术用于婴幼儿ToRCH病原体抗体检测的评估

Determinations of infant ToRCH specific antibodies by multiplex flow immunoassay

目的评价液相悬浮芯片技术检测婴幼儿To RCH病原体[弓形虫(TOX)、风疹病毒(RUB)、人巨细胞病毒(CMV)、单纯疱疹病毒(HSV)]抗体与传统方法的一致性。方法使用液相悬浮芯片技术对130例患儿的To RCH病原体的IgM和IgG抗体进行测定,并与化学发光法进行比对研究,比对不一致的样本进一步采用酶联免疫吸附试验(ELISA)进行对比。结果除抗CMV IgG抗体外,液相悬浮芯片技术的测定结果与化学发光法的一致率均在80.00%以上,二者具有良好的一致性(K>0.4)。而液相悬浮芯片技术检测抗CMV IgG抗体与化学发光法和ELISA的一致率分别仅为45.37%和47.22%,该方法与传统方法(化学发光法和ELISA)缺乏一致性(K值分别为0.10和0.11)。结论液相悬浮芯片技术在测定TOX抗体(IgM和IgG)、RUB抗体(IgM和IgG)、CMV抗体(IgM)和HSV抗体(IgM和IgG)时具有良好的一致性,而在进行抗CMV IgG抗体的测定时与化学发光法和ELISA均有较大差异。

Objective To evaluate the consistency of multiplex flow immunoassay and traditional methods in the determinations of infant ToRCH specific antibodies [toxoplasma（TOX）,rubella（RUB）,cytomegalovirus （CMV） and herpes simplex virus（HSV）].Methods ToRCH-IgM and ToRCH-IgG antibodies were determined by multiplex flow immunoassay in 130 infants,and the results were compared with those of chemiluminescence. The samples with inconsistent results were further determined by enzyme-linked immunosorbent assay（ELISA）. Results Except for CMV IgG antibody,multiplex flow immunoassay and chemiluminescence had good consistency,and the consistency rate was ＆gt;80.00%（〉0.4）. However,for CMV IgG antibody,the consistency rates of multiplex flow immunoassay with chemiluminescence and ELISA were 45.37% and 47.22%（K=0.10 and 0.11）.Conclusions There is a good consistency for TOX antibodies（IgM and IgG）,RUB antibodies（IgM and IgG）,CMV antibody（IgM）and HSV antibodies（IgM and IgG） between multiplex flow immunoassay and traditional methods（chemiluminescence and ELISA）,while there is difference for CMV antibody（IgG）between chemiluminescence and ELISA.