上海地区全血细胞计数结果可比性和一致性调查

Survey on the comparability and consistency of whole blood cell count determination in Shanghai

目的了解上海地区全血细胞计数结果的可比性和一致性。方法组织上海市不同级别医疗机构临床实验室进行全血细胞计数结果调查,分析不同参数检测结果的变异系数(CV)和仪器间的结果差异。结果612家医疗机构实验室使用12个品牌56个型号的血液分析仪检测并上传了检测结果;2个批号调查品不同参数检测结果的CV分别为白细胞(WBC)计数3.34%和3.56%;红细胞(RBC)计数1.46%和1.50%;血红蛋白(Hb)1.42%和1.31%;血细胞比容(HCT)2.89%和2.79%;血小板(PLT)计数3.96%和3.65%。2个批号调查品在不同仪器组检测结果均值(x)与所有仪器组总x差异WBC计数分别为0.38%^-2.25%和0.00%^-2.87%,RBC计数分别为0.13%^-3.47%和0.05%~3.04%,Hb分别为0.16%^-1.48%和0.16%^-1.11%,HCT分别为0.16%^-4.65%和0.05%~4.10%,PLT计数分别为0.15%^-5.43%和-0.27%~3.21%。结论上海地区全血细胞计数结果总体趋于可比和一致,但不同仪器组全血细胞计数结果存在差异。

Objective To know the comparability and consistency of whole blood cell count determination in Shanghai. Methods Various clinical laboratories in Shanghai were enrolled. For the comparability and consistency of whole blood cell count determination,their coefficients of variation（CV）and the difference among different instruments were evaluated. Results A total of 612 clinical laboratories finished the determinations and uploaded their results,and there involves 12 brands and 56 types of hematology analyzers. For 2 batches of investigation products,the CV of white blood cell（WBC） count were 3.34% and 3.56%,those of red blood cell（RBC） count were 1.46% and 1.50%,those of hemoglobin（Hb） were 1.42% and 1.31%,those of hematocrit（HCT） were 2.89% and 2.79%,and those of platelet（PLT） count were 3.96% and 3.65%. The difference between the means （x） of different instruments and total mean were 0.38%--2.25% and 0.00%--2.87% for WBC count,0.13%--3.47% and 0.05%-3.04% for RBC count,0.16%--1.48% and 0.16%--1.11% for Hb,0.16%--4.65% and 0.05%-4.10% for HCT,and 0.15%--5.43% and-0.27%-3.21% for PLT count. Conclusions The results of whole blood cell count determination has comparability and consistency. However,the difference still exists among different instruments.