生长分化因子11对2型糖尿病小鼠胰岛β细胞保护作用的研究

Protective effects of GDF11 on pancreatic β cell in type 2 diabetic mice

目的探讨生长分化因子11(GDF11)对T2DM小鼠胰岛β细胞的作用。方法选取C57BL/6J野生型小鼠,通过高脂饮食及STZ制备T2DM模型并随机均分为糖尿病rGDF11干预组(DM+rGDF11)和糖尿病对照组(DM),同时将普通饲料喂养的C57BL/6J小鼠随机分为正常rGDF11干预组(Con+rGDF11,n=10)和正常对照组(Con,n=10),观察GDF11对小鼠生理生化指标、胰岛β细胞功能及含量指标的影响。结果干预6周后,Con组与Con+rGDF11组各糖脂代谢指标比较,差异无统计学意义(P>0.05)。与DM组比较,DM+rGDF11组FBG、HbA1c、胰升血糖素及血脂水平降低(P<0.05),且rGDF11干预后上调胰岛β细胞重要基因表达,促进胰岛素分泌,改善胰岛形态及β细胞含量(均P<0.05)。结论 GDF11可改善胰岛β细胞功能及维持β细胞含量,进而延缓糖尿病的发生发展。

Objective To investigate the effects of growth differentiation factor 11（GDF11）on pancreaticβcell in type 2 diabetic mouse model. Methods C57 BL/6 Jmice were rendered diabetic by feeding with high fat diet plus intraperitoneal injection of streptozotocin.And then,all the diabetic mice were divided into two groups randomly：DM＋rGDF11 group and DM group.Normal control mice were randomly divided into Con＋rGDF11 group（n=10）and Con group（n=10）.The effects of GDF11 on metabolic parameters,βcell function and βcell mass were evaluated. Results After 6 weeks＇ intervention,the metabolic parameters were not significantly different in Con group and Con＋rGDF11 group（P〈0.05）.FBG,HbA1 c,serum glucagon and lipid profiles were decreased in DM＋rGDF11 group than in DM group（P〈0.05）.rGDF11 administration upregulated expression ofβcell-specific genes,improved insulin secretory response,improved islet morphology andβcell mass（all P 0.05）.Conclusion GDF11 may preserve isletβcell function and mass and thus may delay the progression of diabetes.