长链非编码RNA-BC043614在胰腺导管腺癌中的表达及其临床意义

The expression and clinical significance of the long non-coding RNA-BC043614 in pancreatic ductal adenocarcinoma

目的检测胰腺导管腺癌（PDAC）组织中长链非编码RNA—BC043614（LncRNA。BC043614）的表达，分析其与PDCA患者临床病理特征和预后的关系。方法收集2013年6月至2014年6月在无锡市第二人民医院行胰腺癌根治手术的42例患者的组织样本、临床病理和随访资料。采用实时荧光定量PCR（qRT—PCR）检测癌组织和癌旁组织中LncRNA-BC043614表达水平，X。检验分析其表达量与胰腺癌患者临床病理特征的关系，应用Log—rank检验和COX回归模型分析其与胰腺癌患者手术预后的关系。结果LncRNA—BC043614在癌组织的表达高于癌旁组织（P〈0．05）；PDCA组织中LncRNA．BC043614的表达量与肿瘤大小、分化程度、有无脉管癌栓、患者血清CA19-9相关（P〈0．05）；LncRNA—BC043614高表达患者术后无瘤生存时间明显缩短（P〈0．05），胰腺癌组织中LncRNA—BC043614高表达是影响胰腺癌患者术后无瘤生存期的独立危险因素（P〈0．05）。结论LncRNA—BC043614在胰腺癌组织中高表达，与患者术后无瘤生存期存在相关性，可能成为判断PDCA患者预后的标志物。

Objective To study the expression of the long non-coding RNA-BC043614 in pancreatic dnctal adenocarcinoma （PDAC） and to analyse the relationship between the clinical pathological data and the prognosis of PDAC. Methods The clinical data of 42 patients who had PDAC treated from June 2013 to June 2014 were retrospectively analyzed. Real-time fluorescence quantitative PCR （qRT-PCR） was per- formed to detect the difference in expression of lncRNA-BC043614 between PDAC and its adjacent normal tissues. Correlation between the expression of lncRNA-BC043614 in PDAC and the clinical pathological characteristics was analyzed by the Xz test. The potential of lncRNA-BC043614 in predicting prognosis in pa- tient with PDAC after operation was assessed using the Log-rank test and the Cox＇ s proportional hazards re- gression model. Results The expression of lncRNA-BC043614 was markedly higher in PDAC than in its adjacent normal tissues （P 〈 0.05 ）. The expression of lncRNA-BC043614 in PDAC was significantly corre- lated with tumor size, tumor differentiation, vascular carcinoma, and CA19-9 serum levels （ P 〈 0.05 ）. Pa- tients with a higher expression of lncRNA-BC043614 in PDAC had a significantly shorter tumor-free survival （ P 〈 O. 05）. A high expression of lncRNA-BC043614 in PDAC was an independent risk factor of tumor-free survival in patients with PDAC after operation （ P 〈 0.05 ）. Conclusions lncRNA-BC043614 was highly expressed in pancreatic cancer tissue and it correlated with the patient ＂s tumor-free survival, lncRNA- BC043614 can be used as a new prognostic biomarker.