2015-2016年成都市某区手足口病病原学检测结果分析

Etiological analysis of hand、foot、mouth disease from Chengdu in a District of Chengdu in the year of 2015-2016

目的了解成都市某区近年手足口病病原学流行特征,为制定本地区有效防控措施提供科学依据。方法收集2015-2016年成都市某区部分手足口病临床诊断病人的咽拭子样本,采用实时荧光定量PCR方法检测手足口病毒核酸。结果 110份手足口病临床诊断病例的标本中,检出肠道病毒核酸阳性85例,总检出率为77.27%,其中EV71检出率为19.09%,CoxA16检出率为18.18%,其他肠道病毒检出率为40.00%。病毒核酸阳性标本以<5岁儿童居多,占97.65%。男童阳性检出率高于女童(P<0.05)。结论 2015-2016年成都市某区<5岁儿童为手足口病高发人群,各型别病毒感染同时存在,没有明显流行优势株。应加强手足口病的病原学检测,掌握流行规律,以保护儿童健康。

Objective The purpose of this study is to investigate the pathogen characteristics of hand, foot, mouth disease （HFMD） in a district of Chengdu in 2015-2016, and provide the scientific evidence for establishing the corresponding preventions and controls. Methods Throat swabs samples were collected from 110 clinically diagnosed HFMD cases in the district. Quantitative Real-time PCR was used to detect nucleic acids of HFMD virus. Results The total positive rate of the enterovirus nucleic acid was 77.27% with 19.09% of enterovirus 71 （EV71）, 18.18% of coxasckievirus A16 （CoxA16）, and 40.00% of other enterovirus, respectively. The main age group of onset was from those with less than 5-year old, accounting for 97.65%. The positive rate from male group was significantly higher than that from female （P 〈 0.05）. Conclusion Children less than 5-year old was more susceptible to HFMD infection in Longquanyi District. Various virus types existed at the same time without predominated types. Pathogen detection of HFMD must be strengthened to obtain epidemic characteristics and protect children＇s health.