血浆维生素D水平和机体抗氧化酶活性在老年脑梗死患者中的关联

Correlation between serum Vitamin D levels and antioxidant enzymes in elderly patients with cerebral infarction

目的评价老年脑梗死患者维生素D水平和抗氧化酶活性之间的关系。方法选取2013年6月至2015年10月在本院神经内科住院的50例老年脑梗死患者作为观察组,另招募50名健康者作为健康对照组。评估2组受试者水果和蔬菜的摄入量及阳光照射时间;采用酶联免疫吸附法检测2组受试者血清25羟基维生素D(25(OH)D、血清超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GPX)活性。采用Spearman秩检验分析维生素D水平和抗氧化酶活性的相关性。结果观察组患者中有30.6%有严重维生素D缺乏症,而对照组仅有11.1%,差异有统计学意义(P<0.05);观察组患者的水果(≥1次/d)和蔬菜(>2次/周)消费量均低于健康对照组,差异均有统计学意义(P<0.05);观察组患者抗氧化酶活性和水果摄入量呈正相关(r=1.58;P=0.02)。脑梗死最有效的风险预测因素是高血压史、高水平低密度脂蛋白和心血管疾病史(OR分别为3.33、3.15和3.14,P<0.05)。脑梗死患者血清25(OH)D浓度与抗氧化酶活性(观察组:r=1.03,P=0.34;健康对照组:r=0.72,P=0.50)和血脂(观察组:r=0.35,P=0.17;对照组:r=0.58,P=0.25)之间无相关性。结论脑梗死患者维生素D缺乏比较严重,水果和蔬菜摄入量较低。水果摄入量与抗氧化酶水平呈正相关,而维生素D水平与抗氧化酶活性无相关性。

Objective To evaluate the relation between the status of Vitamin D and antioxidant enzymes in elderly patients with cerebral infarction .Methods The study subjects were 50 elderly patients with cerebral in-farction admitted to the Department of neurology of our hospital from June 2013 to October 2015 ,and 50 healthy control subjects were recruited .Intake of fruits and vegetables ,exposure of sunlight ,serum lipid profile were de-termined.Concentrationsofserum 25-dihydroxy VitaminD （25（OH）D）,activitiesof serum superoxidedis-mutase（SOD） ,and glutathione peroxidase（GPX） enzymes were evaluated by ELISA method .Spearman rank test was used to analyze the correlation between vitamin D levels and antioxidant enzyme activities .Results Severe Vi-tamin D deficiency was seen in 30 .6% of the patients versus 11 .1% of the controls （ P〈0 .05） .Consumption of fruits（≥1 time/day）and vegetables（〉2 times/week） was lower in patients than that of the controls （ P〈0 .05） . Activities of antioxidant enzymes and intake of fruits were positively correlated in cerebral infarction patients （ r=1 .58 ,P =0 .02） .The most potent predictors of cerebral infarction risk were hypertension ,high levels of low-den-sity lipoprotein cholesterol and history of cardiovascular disease （odds ratios ：3 .33 ,3 .15 ,and 3 .14 ,respectively , P 〈 0 .05 for all） .There was no association between 25（OH） D levels with activities of serum antioxidant en-zymes（observation group ：r = 1 .03 ,P =0 .34 ;control group ：r = 0 .72 ,P =0 .50） and lipid profile（observation group：r= 0 .35 ,P=0 .17 ;control group ：r= 0 .58 ,P=0 .25） in the two groups .Conclusion Cerebral infarc-tion patients have higher prevalence of severe Vitamin D deficiency and lower intakes of fruits and vegetables .In-take of fruits was positively correlated to higher antioxidant enzymes levels ,and the level of Vitamin D was not as-sociated with the activity of antioxidant enzymes .