TCDCA对AA模型大鼠成纤维样滑膜细胞中三磷酸肌醇的影响

Effect of TCDCA on Concentration of Inositol Trisphosphate in Fibroblast-like Synoviocytes in Rats with Adjuvant Arthritis

为了进一步探讨牛磺鹅去氧胆酸(TCDCA)对佐剂性关节炎(adjuvant arthritis,AA)模型大鼠治疗作用的分子机制,以AA模型大鼠成纤维样滑膜细胞(fibroblast-like synoviocytes,FLS)为研究对象,采用酶联免疫吸附技术(enzyme linked immune sorbent assay,ELISA)检测了TCDCA对AA模型大鼠FLS中三磷酸肌醇(inositol trisphosphate,IP3)含量的影响。研究结果显示,与空白对照组相比较,TCDCA作用15 min和60 min时AA模型大鼠FLS中IP3的含量显著升高(P<0.05),而当TCDCA作用30 min时AA模型大鼠FLS中IP3的含量极显著升高(P<0.01)。由此可知,TCDCA能够提高AA模型大鼠FLS中IP3的含量。

The aim of the present study was to unravel the molecular mechanism underlining the therapeutical effect of TCDCA on adjuvant arthritis （AA） model rats. The intervention effect of TCDCA on inositol trisphosphate （IP3 ） concentration in fibroblast-like synoviocytes （FLS） of rats with AA was assessed quantitatively by using enzyme linked immunesorbent assay （ELISA）. The results showed that the IP3 concentration was found significantly increased in FLS of AA model rat at15 min （P〈0.05）, 30 min （P〈0.01） and 60 min （P〈0.05） after TCDCA treatment compared to control. The up-regulation of IP3 expression in FLS of rats with AA induced by TCDCA was observed in this study.