紫外可见分光光度法测定瑞香狼毒涂搽剂中伞形花内酯的含量

Determination of Umbelliferone Content in Stellera chamaejasme Liniment by Ultraviolet-visible Spectrophotometry Method

[目的]建立紫外可见分光光度法,测定瑞香狼毒涂搽剂中伞形花内酯的含量。[方法]以中国药品生物制品检定所鉴定的伞形花内酯作为标准品,以伞形花内酯标准品从190~400 nm的最高吸收峰波长323 nm作为测定波长,采用紫外可见分光光度法,测定其吸光度绘制标准曲线。[结果]在0~0.20μg/mL范围内浓度与吸光度线性关系良好(R^2=0.9995),精密度RSD为0.00868%,稳定性RSD为0.101%,重复性RSD为0.305%,回收率为98.04%~100.39%。[结论]该方法准确、快速、重复性好,可用于瑞香狼毒涂搽剂中伞形花内酯的含量测定。

[Objective]To develop an ultraviolet-visible spectrophotometry method for estimation of umbelliferone in a self-prepared pharmaceutical liniment of Stellera chamaejasme. [Methods]Using the umbelliferone identified by National Institutes for Food and Drug Control as standard substance, the absorbance value of samples was determined under wavelength of 323 nm which was screened in the range of 190-400 nm and had the highest absorption peak, and subsequently the standard curve of the ultraviolet -visible spectrophotometry method was drawn. [Results]In the range of 0-0.20 滋 g/mL, there was a good linear relationship between the concentration of umbelliferone and absorbance （R2=0.9995）; the RSD for precision, stability and repeatability was 0.00868%, 0.101% and 0.305%, respectively, and the recovery rate was ranged from 98.04% to 100.39%. [Conclusion]The precise, rapid and repeatable ultraviolet-visible spectrophotometry method developed in this study can be applied in determination of umbelliferone content in Stellera chamaejasme liniment.