miR-190的表达下调有利于抑制结直肠癌

Downregulation of miR-190 Expression is Beneficial in Inhibiting Colorectal Cancer

研究miR-190在结直肠癌(colorectal cancer,CC)患者中的表达和作用机制。采用Real-time PCR和探针原位杂交的方法检测miR-190在结直肠癌患者的癌组织和癌旁正常组织中的表达量变化。利用Targetscan数据库,寻找潜在的miR-190靶基因,并采用双荧光素酶报告基因验证。结果发现,相比于癌旁正常组织,miR-190在结直肠癌患者的癌组织中表达量显著下降(p<0.000 1)。通过Targetscan数据库找到miR-190可作用于细胞因子IGF-1,双荧光素酶报告基因实验也证实了miR-190可以作用于IGF-1的3'UTR区域,从而抑制IGF-1的表达。在结直肠癌的发病过程中,miR-190表达量下降,导致IGF-1含量上升,进而促进了结肠癌的发展,miR-190具有抑癌作用。

The object of this study was to investigate the expression pattern and function ofmiR-190 in patients with colorectal cancer. The expression levels of miR-190 in colorectal cancer samples and adjacent normal tissues were investigated by Real-Time PCR and in situ hybridization. The potential target genes ofmiR-190 were searched from Targetscan database and then validated by dual-luciferase report gene. The results showed that the expression of miR- 190 was significantly decreased in colorectal cancer samples compared to adjacent normal tissues （p 〈0.000 1）. Through Targetscan database, it was found that miR-190 could act on cytokine IGF-1. Also, dual-luciferase report gene test validated that miR-190 could act on the 3＇UTR region of IGF-1 so as to inhibit the expression of IGF-1. In the process of colorectal cancer, the expression of miR-190 decreased, leading to an increase of IGF-1 and promo- tion of colorectal cancer development. Therefore, miR-190 could potentially inhibit colorectal cancer.