小鼠心肌层注射方法的改进

Improvement of myocardial layer injection in mice

探索心肌有效稳定的给药方式,达到优化手术的均一性和可重复性.采用改良后的注射针头,分别向小鼠心肌层注射伊文思蓝(Evans Blue)和带绿色荧光蛋白的慢病毒,结果发现伊文思蓝和慢病毒能够全部存留在心肌中,而且慢病毒能够在整个心脏中稳定长期地表达绿色荧光蛋白.研究表明,改良的心肌层注射方式均一性好,手术成功率高,可用于心血管研究和基因治疗.

An improved myocardial layer injection acts as an effectual mode of adminis- tration was established. Used improved syringe, Evans Blue and green fluorescent protein （CFP） labeled lentivirus were injected in to mouse myocardial layer. Evans Blue and lentivirus were found to be stored in mouse myocardial layer, and GFP could stably ex- press in the whole heart for a long time. In conclusion, an improved myocardial layer injection may be used to cardiovascular disease （CVD） research and gene therapy research because of the good uniformity and high success rate.