GATA3-IL-13通路基因多态性和IL-13水平与哮喘病的相关性研究

GATA3-IL-13 pathway gene polymorphisms are associated with serum IL-13 and asthma

目的:哮喘是一种慢性炎症性呼吸道疾病,有数据表明GATA3-IL-13基因参与哮喘病的生物学理论是可信的。本研究目标是调查哮喘患者中GATA3-IL13通路基因的多态性与IL-13水平的关系,评估在新疆人口中GATA3-IL-13基因多态性与IL-13水平的相关性。方法:279例哮喘患者和277例对照组(健康人)通过Mass ARRAY SNP分型系统进行分析,通过ELISA法检测IL-13的水平,运用SPSS22.0和Graph Pad Prism 6.0软件进行数据统计和分析。结果:哮喘组IL-13水平中(rs2066960 AA),GATA3(rs3781093 CC)基因型与CC(rs2066960)、TT(rs3781093)相比具有增加患病的风险(P<0.05)。同样的,IL-13(rs2066960)C-A等位基因具有增加哮喘患病的风险(P<0.05)。而rs3781093 C-T等位基因没有显著性差异(P>0.05)。结论:哮喘组GATA3(rs3781093)风险性位点发现与哮喘病相关,rs2066960等位基因的C-A突变与血清中IL-13水平的表达有关。

Objective： Asthma is a chronic inflammatory respiratory disease. The data show that involvement of the GATA3-IL-13 gene in asthma is biologically plausible. The objective of this study is investigated the association of GATA3-IL13 gene polymorphisms and IL-13 levels with asthma; assess the correlations between GATA3-IL-13 gene SNPs polymorphisms and serum levels of IL-13 in population of Xinjiang（ China）. Methods： A case group of 279 patients and 277 healthy controls were genotyped to perform using the Mass ARRAY SNP genotyping system. In 279 asthma patients and 277 controls,IL-13 levels were measured by ELISA. Data were analyzed using the statistical package for social science（ SPSS） 22. 0（ IBM,NY,USA） and Graph Pad Prism 6. 0. Results： Patients were found that IL-13 levels were associated with asthma in asthmatic and the IL-13（ rs2066960 AA）,GATA3（ rs3781093 CC） genotype was associated with a notably increased risk of asthma compared with the CC（ rs2066960）,TT（ rs3781093） genotype（ P〈0. 05）. Similarly,IL-13（ rs2066960） C-A alleles were significantly associated with risk of asthma（ P〈0. 05）. However,the rs3781093 C-T alleles had no obvious differences（ P〉0. 05）. In addition,the patients carrying the rs2066960 AA genotype presented with higher IL-13 levels compared to the CC group. Conclusion： This result suggests that the rs2066960 C-A variant is associated with IL-33 levels in patients with asthma.