人工打洞沉香中1个新的倍半萜

A new sesquiterpene from Chinese agarwood induced by artificial holing

运用多种色谱技术,对人工打洞沉香乙醇提取物的正丁醇相进行化学成分分离,并根据波谱数据结合理化性质共鉴定了7个单体化合物的结构,分别为selina-3,11-dien-9,15-diol(1)、aquilarone D(2)、5α,6β,7α,8β-tetrahydroxy-2-[2-(2-hydroxyphenyl)ethyl]-5,6,7,8-tetrahydrochromone(3)、6,7-dimethoxy-2-[2-(4-methoxyphenyl)ethyl]chromone(4)、syringin(5)、顺式对羟基肉桂酸甲酯(6)和4'-甲氧基肉桂酸(7),其中化合物1为新的倍半萜类化合物,化合物5~7为首次从沉香中分离得到。生物活性测试结果表明化合物6,7对全齿复活线虫具有较好的致死活性,化合物4,6,7对人肝癌细胞株BEL-7402、人胃癌细胞株SGC-7901和人肺癌细胞株A549的生长有不同程度的抑制作用。

In order to study the chemical constituents of n-butanol fraction of ethanol extract from Chinese agarwood induced by arti- ficial holing, various chromatographic techniques were carried out to isolate compounds, and the structures of compounds were deter- mined through a combined analysis of physicochemical properties and spectroscopic evidence. Seven compounds were obtained and i- dentified as selina-3,11 -dien-9,15-diol （ 1 ）, aquilarone D （ 2 ）, 5a, 6/3,7 a, 8/3-tetrahydroxy-2 - [ 2 - （ 2 -hydroxyphenyl ） ethyl ] -5,6,7,8- tetrahydrochromone （3）, 6,7-dimethoxy-2 - [ 2- （4 -methoxyphenyl） ethyl~ chromone （ 4 ）, syringin （ 5 ）, methyl （Z） -p -coumarate （ 6 ）, and 4＇-methoxycinnamic acid （7） , among which compound 1 was a new compound and compounds 5-7 were isolated from agarwood for the first time. The bioactivity assay results concluded that compounds 6 and 7 showed certain nematicidal activity against PanagreUusredivivus, and compounds 4, tl and 7 exhibited cytotoxicity against BEL-7402, SGC-7901 and A549 carcinoma cell lines.