基于多波长HPLC-DAD比较栀子不同部位化学成分差异

Comparison of chemical constituents in different parts of Gardenia jasminoides based on multiple wavelength HPLC-DAD

采用多波长HPLC-DAD,同时测定栀子不同部位去乙酰车叶草酸甲酯、羟异栀子苷、栀子苷、绿原酸、西红花苷Ⅰ、西红花苷Ⅱ和竹节参皂苷Ⅳa 7种化合物的含量,比较各成分在栀子不同部位的分布。结果表明栀子中各成分在各部位分布不同,且含量存在较大差异,其中栀子苷主要分布在果实和叶中,竹节参皂苷IVa主要分布于根和茎中,西红花苷类成分主要存在于果实中,绿原酸以叶和茎含量较高,羟异栀子苷在叶和根中含量较高,去乙酰车叶草酸甲酯在根和茎中含量较高。通过分析栀子不同部位化学成分含量差异,为栀子资源综合开发利用和质量评价提供依据。

Seven compounds （deacetylasperulasidic acid methyl ester, gardenoside, chlorogenic acid, geniposide, crocin-I , cro- cin-Ⅱ, chikusetsu saponin IVa）were determined simultaneously by multiple wavelength HPLC with diode array detector（DAD） in dif- ferent parts of Gardenia jasminoides. The results showed that these components in different parts of G. jasminoides had a different distri- bution, and there was a large difference in content of each component. Geniposide was mainly distributed in fruits and leaves; chiku- setsu saponin 1Va was mainly distributed in roots and stems; crocus glycosides existed mainly in fruits; chlorogenic acid had a higher distribution in leaves and stems; gardenoside had a higher distribution in leaves and roots, while ceacetylasperulasidie acid methyl ester had a higher distribution in roots and stems. Based on the analysis of the chemical composition and content difference in different parts of G. ]asminoides, the basis for the comprehensive utilization and quality evaluation of resources of G. jasminoides was provided.