HPLC法测定鬼针草不同部位三种黄酮成分含量

Determination of Three Flavonoids in Different Parts of Bidens bipinnata L. by HPLC

目的 :建立同时测定鬼针草中三种黄酮类成分含量的方法 ,并比较鬼针草茎和叶中三种成分的含量。方法 :采用HPLC法对鬼针草中三种黄酮类成分芦丁、异奥卡宁7-0-β-D-葡糖苷和异槲皮苷的含量测定方法学进行考察,并同时分析三种黄酮类成分在鬼针草茎和叶总黄酮中的含量。结果:芦丁在0.010~1.040 mg/m L(r=0.999 9)、异奥卡宁7-0-β-D-葡糖苷在0.012~1.168 mg/m L(r=0.999 5)、异槲皮苷在0.012~1.230 mg/m L线性关系良好;平均回收率(n=6)分别为98.51%、99.21%、98.73%、97.33%、98.92%、98.77%。采用此方法同时测定鬼针草茎和叶中芦丁、异奥卡宁7-0-β-D-葡糖苷、异槲皮苷三种黄酮的含量。结论:建立的HPLC法同时测定鬼针草中三种黄酮类成分含量的方法良好,可用于鬼针草不同部位中黄酮的含量测定。

Objective：To establish the method to determine the contents of three flavonoids in the leaves as well as in the stems of Bidens bipinnata L.simultaneously.Methods：HPLC was used to investigate the method for determining the contents of three flavonoids including：rutin,iso-okanin-7-O-β-D-glucoside,isoquercitri.Results：The methodological evaluation showed this method had good linearities within the test ranges and had good recoveries.The calibration curves of rutin,iso-okanin-7-O-β-D-glucoside,isoquercitrin were linear in the range of 0.010~1.040 mg/m L（r= 0.999 9）,0.012~1.168 mg/m L（r= 0.999 5）,0.012~1.23 mg/m L（r= 0.999 6）respectively.The average recoveries（n= 6） of the three components from the samples were 98.51%,99.21%,98.73%,97.33%,98.92%,98.77% respectively.And the contents of rutin,iso-okanin-7-O-β-D-glucoside,isoquercitrin in the leaves and stems of Bidens bipinnata L.were determined simultaneously.Conclusion：The HPLC method can be used for the determination of the flavonoids in the different parts of Bidens bipinnata L.with good effects.